PPP2R3A antibody (AA 1073-1085)

Catalog No. ABIN337311

Product Details anti-PPP2R3A Antibody

Target: PPP2R3A (Protein Phosphatase 2, Regulatory Subunit B'', alpha (PPP2R3A))

Binding Specificity: AA 1073-1085

Reactivity: Human, Mouse, Horse, Rabbit, Monkey, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This PPP2R3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PPP2R3A. This antibody is expected to recognise both reported isoforms (NP_002709.2, NP_871626.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Horse, Rabbit, Pig, Lizard (100%) Rat, Hamster, Bat, Bovine, Opossum, Turkey, Chicken (92%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QKDVENDGPEPSD from an internal region of human PPP2R3A (NP_002709.2, NP_871626.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Elephant, Horse, Rabbit, Pig, Lizard (100%), Rat, Hamster, Bat, Bovine, Opossum, Turkey, Chicken (92%), Dog (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:16000), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PPP2R3A

Target: PPP2R3A (Protein Phosphatase 2, Regulatory Subunit B'', alpha (PPP2R3A))

Alternative Name: PPP2R3A / PR130 (PPP2R3A Products)

Synonyms: PPP2R3 antibody, PR130 antibody, PR72 antibody, 3222402P14Rik antibody, A730042E07 antibody, PPP2R3A antibody, Ppp2r3a antibody, protein phosphatase 2 regulatory subunit B''alpha antibody, protein phosphatase 2, regulatory subunit B'', alpha antibody, protein phosphatase 2 regulatory subunit B, alpha S homeolog antibody, serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha antibody, PPP2R3A antibody, Ppp2r3a antibody, ppp2r3a.S antibody, LOC100088744 antibody, LOC100478108 antibody, ppp2r3a antibody, LOC100547843 antibody, LOC100729010 antibody

Background: Name/Gene ID: PPP2R3A

Synonyms: PPP2R3A, PR130, PR72, PPP2R3, PP2A, subunit B, R3 isoform

Gene ID: 5523

NCBI Accession: NP_002709, NP_871626

UniProt: Q06190

Pathways: PI3K-Akt Signaling