Arylsulfatase D antibody (AA 108-120)

Catalog No. ABIN343011

Product Details anti-Arylsulfatase D Antibody

Target: Arylsulfatase D (ARSD)

Binding Specificity: AA 108-120

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Arylsulfatase D antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Specificity: Human ARSD / Arylsulfatase D. This antibody is expected to recognise both reported isoforms (NP_001660.2, NP_033667.2)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%) Marmoset (92%) Opossum, Platypus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DASNGYRALQWNA from an internal region of human ARSD / Arylsulfatase D (NP_001660.2, NP_033667.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Marmoset (92%), Opossum, Platypus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:16000, 0.3 - 1 μg/mL), WB (0.3 - 1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx 60+40kD bands observed in lysates of cell lines A549 and HepG2 (calculated MW of 64.9kD according to NP_001660.2 and of 42.3 according to NP_033667.2). Recommended concentration: 0.3-1 μg/mL. An additional band of unknown identity was also consistently observed at 35kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Arylsulfatase D

Target: Arylsulfatase D (ARSD)

Alternative Name: ARSD / Arylsulfatase D (ARSD Products)

Synonyms: ASD antibody, ARSD antibody, MGC81982 antibody, arylsulfatase D antibody, arylsulfatase D L homeolog antibody, ARSD antibody, arsd.L antibody, LOC516494 antibody, arsd antibody, BMEA_B1180 antibody, LOC100624032 antibody

Background: Name/Gene ID: ARSD

Synonyms: ARSD, Arylsulfatase D, ASD

Gene ID: 414

NCBI Accession: NP_001660, NP_033667

UniProt: P51689