APOLD1 antibody (AA 260-273)

Catalog No. ABIN343090

Product Details anti-APOLD1 Antibody

Target: APOLD1 (Apolipoprotein L Domain Containing 1 (APOLD1))

Binding Specificity: AA 260-273

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOLD1 antibody is un-conjugated

Application: ELISA

Specificity: Human APOLD1 / Apolipoprotein L D1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%) Marmoset, Bovine (93%) Panda, Bat, Rabbit (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KSSRGHDLKISADQ from the C-terminus of human APOLD1 / Apolipoprotein L D1 (NP_110444.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Marmoset, Bovine (93%), Panda, Bat, Rabbit (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:2000)

Usage: Peptide ELISA: antibody detection limit dilution 1:2000. Western Blot: Preliminary experiments gave an approx. 37kD band in Human Brain (Frontal Cortex) lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.2kD according to NP_110444.1 The 37kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for APOLD1

Target: APOLD1 (Apolipoprotein L Domain Containing 1 (APOLD1))

Alternative Name: APOLD1 / Apolipoprotein L D1 (APOLD1 Products)

Synonyms: Gm1075 antibody, APOLD1 antibody, MGC145863 antibody, VERGE antibody, Verge antibody, apolipoprotein L domain containing 1 antibody, Apold1 antibody, APOLD1 antibody, apold1 antibody

Background: Name/Gene ID: APOLD1

Synonyms: APOLD1, DKFZP434F0318, VERGE

Gene ID: 81575

NCBI Accession: NP_110444

UniProt: Q96LR9