Septin 7 antibody (AA 322-334)

Catalog No. ABIN343122

Product Details anti-Septin 7 Antibody

Target: Septin 7 (SEPT7)

Binding Specificity: AA 322-334

Reactivity: Human, Rat, Mouse, Cow, Horse, Dog, Chicken, Xenopus laevis, Pig, Monkey, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This Septin 7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human SEPT7 / Septin 7. This antibody is expected to recognize all three reported isoforms (NP_001779.3, NP_001011553.2, NP_001229885.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Horse, Pig, Chicken, Xenopus, Stickleback (100%) Zebrafish (92%) Salmon (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DNNKNKGQLTKSP from an internal region of human SEPT7 / Septin 7 (NP_001779.3, NP_001011553.2, NP_001229885.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Bovine, Horse, Pig, Chicken, Xenopus, Stickleback (100%), Zebrafish (92%), Salmon (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:32000, 0.3 - 1 μg/mL), WB (0.3 - 1 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 45kD band observed in nuclear lysates of cell line HeLa (calculated MW of 50.7kD according to NP_001779.3 and of 55.6kD according to NP_001011553.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Septin 7

Target: Septin 7 (SEPT7)

Alternative Name: SEPT7 / Septin 7 (SEPT7 Products)

Synonyms: cdc10 antibody, wu:fb38h06 antibody, zgc:56383 antibody, Septin-7 antibody, CDC10 antibody, CDC3 antibody, NBLA02942 antibody, SEPT7A antibody, Cdc10 antibody, E430034N22 antibody, septin 7a antibody, septin 7 antibody, septin 7 L homeolog antibody, sept7a antibody, SEPT7 antibody, sept7.L antibody, sept7 antibody, Sept7 antibody

Background: Name/Gene ID: SEPT7

Synonyms: SEPT7, CDC10, CDC10 protein homolog, Hcdc10, NBLA02942, Sep7, SEPT7A, Septin 7, Septin-7, CDC3

Gene ID: 989

NCBI Accession: NP_001779, NP_001011553, NP_001229885

UniProt: Q16181