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CD34 antibody

CD34 Reactivity: Human, Non-Human Primate FACS, WB, IHC (p), IP, IHC (fro), Func Host: Mouse Monoclonal QBEnd-10 unconjugated
Catalog No. ABIN343702
  • Target See all CD34 Antibodies
    CD34
    Reactivity
    • 300
    • 105
    • 104
    • 33
    • 19
    • 17
    • 17
    • 17
    • 16
    • 16
    • 9
    • 6
    • 5
    • 1
    • 1
    Human, Non-Human Primate
    Host
    • 169
    • 145
    • 17
    • 4
    Mouse
    Clonality
    • 216
    • 116
    • 2
    Monoclonal
    Conjugate
    • 127
    • 28
    • 24
    • 21
    • 14
    • 8
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD34 antibody is un-conjugated
    Application
    • 233
    • 202
    • 90
    • 64
    • 58
    • 52
    • 39
    • 37
    • 29
    • 28
    • 23
    • 20
    • 13
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)
    Specificity
    The antibody QBEnd-10 reacts with an extracellular class II epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells, it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.
    No Cross-Reactivity
    Cow, Dog, Rat, Sheep
    Cross-Reactivity (Details)
    Human, Non-Human Primates
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Human endothelial vesicles
    Clone
    QBEnd-10
    Isotype
    IgG1
    Top Product
    Discover our top product CD34 Primary Antibody
  • Application Notes
    Functional application: The antibody QBEnd-10 induces homotypic adhesion of leukemic cell line.
    Flow cytometry: Recommended dilution: 5 μg/mL.
    Immunohistochemistry (paraffin sections): Recommended dilution: 2-8 μg/mL.
    Western blotting: Recommended dilution: 1-2 μg/mL, positive control: TF-1 cells.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4
    Preservative
    Azide free
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Poblet, Jimenez-Acosta, Rocamora: "QBEND/10 (anti-CD34 antibody) in external root sheath cells and follicular tumors." in: Journal of cutaneous pathology, Vol. 21, Issue 3, pp. 224-8, (1994) (PubMed).

    Traoré, Hirn: "Certain anti-CD34 monoclonal antibodies induce homotypic adhesion of leukemic cell lines in a CD18-dependent and a CD18-independent way." in: European journal of immunology, Vol. 24, Issue 10, pp. 2304-11, (1994) (PubMed).

    Kuzu, Bicknell, Harris, Jones, Gatter, Mason: "Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours." in: Journal of clinical pathology, Vol. 45, Issue 2, pp. 143-8, (1992) (PubMed).

    Sutherland, Marsh, Davidson, Baker, Keating, Mellors: "Differential sensitivity of CD34 epitopes to cleavage by Pasteurella haemolytica glycoprotease: implications for purification of CD34-positive progenitor cells." in: Experimental hematology, Vol. 20, Issue 5, pp. 590-9, (1992) (PubMed).

    Ramani, Bradley, Fletcher: "QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections." in: Histopathology, Vol. 17, Issue 3, pp. 237-42, (1991) (PubMed).

    Schlingemann, Rietveld, de Waal, Bradley, Skene, Davies, Greaves, Denekamp, Ruiter: "Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 62, Issue 6, pp. 690-6, (1990) (PubMed).

  • Target
    CD34
    Alternative Name
    CD34 (CD34 Products)
    Synonyms
    AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody
    Background
    CD34 Molecule,CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
    Gene ID
    947
    UniProt
    P28906
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