AGR3 antibody

Catalog No. ABIN343711

Product Details anti-AGR3 Antibody

Target: AGR3 (Anterior Gradient 3 (AGR3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AGR3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Specificity: The antibody AGR3.1 recognizes the epitope HETTDKNLS within the AGR3 (AG3) protein (19-20 kDa), a secreted cytoplasmic protein which can serve as a marker of carcinogenesis.

Cross-Reactivity (Details): Human, Other species Not tested

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Purified human AGR3 protein

Clone: AGR3-1

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 1 μg/mL, positive tissue: breast cancer.
Immunohistochemistry (frozen sections): Recommended dilution: 1 μg/mL, positive tissue: breast cancer.
Western blotting: Recommended dilution: 1 μg/mL, positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.
Immunocytochemistry: Recommended dilution: 1 μg/mL, positive control: T47D breast cancer cell line, negative control: H1299 lung carcinoma cell line.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for AGR3

Target: AGR3 (Anterior Gradient 3 (AGR3))

Alternative Name: AGR3 (AGR3 Products)

Synonyms: AG3 antibody, BCMP11 antibody, HAG3 antibody, PDIA18 antibody, hAG-3 antibody, E030025L21Rik antibody, Gm888 antibody, RGD1565406 antibody, anterior gradient 3, protein disulphide isomerase family member antibody, anterior gradient 3 antibody, AGR3 antibody, Agr3 antibody

Background: Anterior gradient 3, protein disulphide isomerase,AGR3 (Anterior Gradient 3) protein, also known as AG3 (hAG-3, HAG3 in human), or BCMP11, is a secreted cytoplasmic protein which is involved in metastasis induction and p53 tumour supressor inhibition. It may serve as molecular marker and potential therapeutic target for hormone-responsive breast tumours. Its Xenopus homolog is associated with anteroposterior fate determination during early development.,AG3, HAG3

Gene ID: 155465

UniProt: Q8TD06