PADI3 antibody (N-Term)

Catalog No. ABIN347067

Product Details anti-PADI3 Antibody

Target: PADI3 (Peptidyl Arginine Deiminase, Type III (PADI3))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PADI3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: Reacts with 75 kDa PADI3 protein from mouse, human and rat

Cross-Reactivity (Details): No cross reaction with the other isoforms.

Purification: Antiserum

Immunogen: A 15 residue synthetic peptide derived from N-terminal domain of human PADI3 protein

Isotype: IgG

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
ELISA(1:5000 - 1:20000) IHC / IF(1:50 - 1:500) IP(1:20 - 1:100) WB(1:200 - 1:2000)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

References for anti-PADI3 antibody (ABIN347067)

Young, Rothfuss, Gard, Muth, Thompson, Ashley, Cherrington: "Citrullination regulates the expression of insulin-like growth factor-binding protein 1 (IGFBP1) in ovine uterine luminal epithelial cells." in: Reproduction (Cambridge, England), Vol. 153, Issue 1, pp. 1-10, (2018) (PubMed).

Khan, Edwards, Muth, Thompson, Cherrington, Navratil: "GnRH stimulates peptidylarginine deiminase catalyzed histone citrullination in gonadotrope cells." in: Molecular endocrinology (Baltimore, Md.), pp. me20161085, (2016) (PubMed).

Li, Hayward, Jenkins, Rothfuss, Young, Nevalainen, Muth, Thompson, Navratil, Cherrington: "Peptidylarginine Deiminase 3 (PAD3) Is Upregulated by Prolactin Stimulation of CID-9 Cells and Expressed in the Lactating Mouse Mammary Gland." in: PLoS ONE, Vol. 11, Issue 1, pp. e0147503, (2016) (PubMed).

Target Details for PADI3

Target: PADI3 (Peptidyl Arginine Deiminase, Type III (PADI3))

Alternative Name: Protein-Arginine Deiminase Type-3 (PADI3 Products)

Synonyms: cPAD3 antibody, PADI3 antibody, PAD3 antibody, PDI3 antibody, AI507004 antibody, Pad3 antibody, Pdi3 antibody, mKIAA4171 antibody, PAD antibody, peptidyl arginine deiminase-like antibody, peptidyl arginine deiminase 3 antibody, protein-arginine deiminase type-3 antibody, peptidyl arginine deiminase, type III antibody, PADIL antibody, PADI3 antibody, LOC100017366 antibody, Padi3 antibody

Background: Catalyzes the deimination of arginine residues of proteins. Involved in protein citrullination.

Gene ID: 51702

UniProt: Q9ULW8