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EpCAM antibody (FITC)

EPCAM Reactivity: Human FACS, IF, IHC (fro) Host: Mouse Monoclonal ESA214 FITC
Catalog No. ABIN356311
  • Target See all EpCAM (EPCAM) Antibodies
    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
    Reactivity
    • 283
    • 75
    • 35
    • 2
    • 1
    • 1
    Human
    Host
    • 150
    • 143
    • 10
    • 1
    Mouse
    Clonality
    • 186
    • 114
    • 2
    Monoclonal
    Conjugate
    • 154
    • 20
    • 17
    • 13
    • 9
    • 9
    • 8
    • 8
    • 8
    • 8
    • 8
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This EpCAM antibody is conjugated to FITC
    Application
    • 218
    • 177
    • 123
    • 111
    • 90
    • 33
    • 26
    • 24
    • 23
    • 20
    • 19
    • 16
    • 14
    • 10
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    This antibody has been used recently to characterize the EpCAM high CD44+ versus EpCAM low/Cd44- indifferent cancer cells.
    Cellular Localization: Cell membrane.
    Characteristics
    Synonyms: Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2,M4S1, MIC18, Tumor-associated calcium signal transducer 1, Major gastrointestinaltumor-associated protein GA733-2, Epithelial glycoprotein 314,Adenocarcinoma-associated antigen, KS 1/4 antigen
    Purification
    Protein A Chromatography.
    Immunogen
    Small cell lung carcinoma. Remarks: Molecular weight of antigen: 40 kDa.
    Clone
    ESA214
    Isotype
    IgG1
    Top Product
    Discover our top product EPCAM Primary Antibody
  • Application Notes
    Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. Antigen retriever like pepsin enhances the staining of FFPE tissue. Immunofluorescence: 10-20 μg/mL (1/10-1/20), incubate for 2 hours in the dark at RT or itcan also be incubated overnight at 4 °C. Flow Cytometry: 0.2-1.0 μg/0.1 mL(1/200-1/1,000). Recommended Positive Control: Breast carcinoma.
    Restrictions
    For Research Use only
  • Concentration
    0.2 mg/mL
    Buffer
    PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
    Shelf life: One year from despatch.
    Expiry Date
    12 months
  • Target
    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
    Alternative Name
    CD326 / EPCAM / TACSTD1 (EPCAM Products)
    Synonyms
    DIAR5 antibody, EGP-2 antibody, EGP314 antibody, EGP40 antibody, ESA antibody, HNPCC8 antibody, KS1/4 antibody, KSA antibody, M4S1 antibody, MIC18 antibody, MK-1 antibody, TACSTD1 antibody, TROP1 antibody, ECS-1 antibody, ECS1 antibody, ESA1 antibody, M17S1 antibody, EGP antibody, Ep-CAM antibody, sb:cb6 antibody, tacstd antibody, wu:fj17g02 antibody, zgc:110304 antibody, zgc:77119 antibody, tacstd1 antibody, MGC80540 antibody, EPCAM antibody, egp antibody, ksa antibody, m4s1 antibody, mk-1 antibody, cd326 antibody, egp40 antibody, mic18 antibody, trop1 antibody, ep-cam antibody, hegp-2 antibody, co17-1a antibody, ga733-2 antibody, CD326 antibody, Egp314 antibody, EpCAM1 antibody, GA733-2 antibody, Ly74 antibody, Tacsd1 antibody, Tacstd1 antibody, gp40 antibody, epithelial cell adhesion molecule antibody, flotillin 2 antibody, epithelial cell adhesion molecule S homeolog antibody, EPCAM antibody, FLOT2 antibody, epcam antibody, epcam.S antibody, Epcam antibody
    Background
    Epithelial is a transmembrane glycoprotein (40 kDa) also known as ESA or epithelial cellular adhesion molecule (Ep-CAM). This protein is expressed on basolateral cell surface in most simple epithelia and vast majority of cancers. According to the Synonyms: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1
    Gene ID
    4072
    UniProt
    P16422
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