Ubiquitin antibody

Catalog No. ABIN356467

Product Details anti-Ubiquitin Antibody

Target: Ubiquitin

Reactivity: Human, Rat, Mouse, Cow, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody stains senile plaques and neurons.
Cellular Localization: Nuclear, Cytoplasmic, and cell membrane.

Purification: Protein A Chromatography

Immunogen: Purified Human Ubiquitin.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 usingstreptavidinapprox. biotin system or polymer system. FFPE tissue sections require antigenretriever, boiling tissue for 15-20 minutes in 10 mM citrate, pH 6.0, followed by cooling atRT for 15-20 minutes. Immunofluorescence. Recommended Positive Control: Human brain from Alzheimer's disease.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 3 mg/mL

Buffer: PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Ubiquitin

Target: Ubiquitin

Alternative Name: Ubiquitin (Ubiquitin Products)

Synonyms: CG11624 antibody, DmUb antibody, DmUbi-p63E antibody, Dmel\\CG11624 antibody, Ub antibody, Ubi-P63e antibody, Ubi-p antibody, Ubi63E antibody, Ubi63p antibody, Ubip antibody, Ubiq antibody, Ubp antibody, ubi antibody, ubi-p63E antibody, ubi63E antibody, CG18282 antibody, CG32744 antibody, CR32744 antibody, DmUbi-p5E antibody, Dmel\\CG32744 antibody, ubiquitin antibody, si:dz180g5.5 antibody, si:busm1-180g5.5 antibody, LOC100194618 antibody, UBIQ antibody, Ubiquitin antibody, LOC100282068 antibody, Ubiquitin-63E antibody, Ubiquitin-5E antibody, transmembrane and ubiquitin-like domain containing 2 antibody, polyubiquitin antibody, Putative ubiquitin family protein antibody, ubiquitin antibody, ubiquitin B antibody, UBiQuitin antibody, Ubiquitin antibody, Ubi-p63E antibody, Ubi-p5E antibody, tmub2 antibody, LOC100194618 antibody, pco110026 antibody, LOC100135798 antibody, UBB antibody, ubq-1 antibody, LOC100190766 antibody

Background: Ubiquitin is a 76 amino acids 8.5 kDa, a highly conserved small regulatory protein that is ubiquitously expressed in eukaryotes and plays a central role in protein degradation. The Ubiquitin/proteosome pathways are main, non-lysomal route for intracellular protein degradation. The protein to be degraded is conjugated to Ubiquitin, it is selectively degraded by 26S complex multicatalytic cytosolic and nuclear protease termed proteosome. This protein also plays important role in cellular processes e.g. Cell cycle progression, gene transcription, antigen processing and signal transduction. In Immunohistochemistry (IHC) this antibody can detect abnormal inclusion bodies, in neurofibrillary tangles in Alzheimer's disease, lewy bodies in Parkinson's disease, pick bodies in Pick's disease, inclusions in motor neuron disease, Mallory's Haylin in alcoholic liver disease and Rosenthal fibers in astrocytes.Synonyms: Polyubiquitin-B, UBB

Gene ID: 7314

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway