PRKAR1B antibody (N-Term)

Catalog No. ABIN359120

Product Details anti-PRKAR1B Antibody

Target: PRKAR1B (Protein Kinase, CAMP-Dependent, Regulatory, Type I, beta (PRKAR1B))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAR1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to PKA R1 beta (PRKAR1B).

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PRKAR1B.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PRKAR1B

Target: PRKAR1B (Protein Kinase, CAMP-Dependent, Regulatory, Type I, beta (PRKAR1B))

Alternative Name: PRKAR1B (PRKAR1B Products)

Synonyms: PRKAR1B antibody, AI385716 antibody, RIbeta antibody, RGD1563094 antibody, PRKAR1 antibody, zgc:153624 antibody, prkar1 antibody, protein kinase cAMP-dependent type I regulatory subunit beta antibody, protein kinase, cAMP dependent regulatory, type I beta antibody, protein kinase cAMP-dependent type 1 regulatory subunit beta antibody, protein kinase, cAMP-dependent, regulatory, type I, beta antibody, protein kinase, cAMP-dependent, regulatory subunit type I beta S homeolog antibody, PRKAR1B antibody, prkar1b antibody, Prkar1b antibody, prkar1b.S antibody

Background: The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93 % amino acid sequence similarity), whereas Cγ displays 83 % and 79 % similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.Synonyms: PKA regulatory subunit I beta, PRKAR1, cAMP-dependent protein kinase type I-beta regulatory subunit

Gene ID: 5575, 9606

UniProt: P31321

Pathways: Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma