EPH Receptor A2 antibody (EPHA2) (AA 38-67)

Details for Product anti-EPHA2 Antibody No. ABIN359790, Supplier: Log in to see
Antigen
  • ARCC2
  • CTPA
  • CTPP1
  • CTRCT6
  • ECK
  • epha2
  • MGC89505
  • AW545284
  • Eck
  • Myk2
  • Sek-2
  • Sek2
  • id:ibd5152
  • rtk6
  • G42
  • XE10
  • epha2-A
  • EPH receptor A2
  • Eph receptor A2
  • epoxide hydrolase
  • eph receptor A2 a
  • EPH receptor A2 L homeolog
  • EPHA2
  • Epha2
  • epha2
  • ephA2
  • epha2a
  • epha2.L
Alternatives
anti-Human EPH Receptor A2 antibody for Flow Cytometry
Epitope
AA 38-67, N-Term
20
19
19
18
15
15
12
12
11
11
11
10
9
9
9
7
7
7
6
5
5
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3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Hamster, Human, Mouse (Murine)
286
171
60
20
14
9
8
7
4
4
2
2
2
2
1
Host
Rabbit
242
66
15
2
2
1
Clonality
Polyclonal
Conjugate
This EPH Receptor A2 antibody is un-conjugated
19
14
14
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12
8
5
5
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4
4
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3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
240
169
89
64
30
27
18
15
7
5
4
4
3
3
2
2
1
1
Options
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Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38~67 amino acids from the N-terminal region of Human EphA2.
Isotype Ig Fraction
Specificity This antibody recognizes EphA2 (N-term)
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Plasmids, Primers & others Plasmids, Primers & others EPH Receptor A2 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name EPHA2 / ECK (EPHA2 Antibody Abstract)
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).Synonyms: Eph receptor A2, Ephrin type-A receptor 2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK
Gene ID 1969, 9606
UniProt P29317
Pathways RTK Signaling
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Flow Cytometry: 1/10-1/50. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
Flow Cytometry (FACS) image for anti-EPH Receptor A2 (EPHA2) (AA 38-67), (N-Term) antibody (ABIN359790) Flow Cytometric analysis of NCI-H292 cells using EphA2 Antibody (N-term) (AP14274PU-N...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EPH Receptor A2 (EPHA2) (AA 38-67), (N-Term) antibody (ABIN359790) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-EPH Receptor A2 (EPHA2) (AA 38-67), (N-Term) antibody (ABIN359790) LEFT: Western blot analysis of hEPHA2-T45 in MCF-7 cell line lysates (35ug/lane). EPH...
Background publications Lindberg, Hunter: "cDNA cloning and characterization of eck, an epithelial cell receptor protein-tyrosine kinase in the eph/elk family of protein kinases." in: Molecular and cellular biology, Vol. 10, Issue 12, pp. 6316-24, 1991 (PubMed).

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