PACSIN1 antibody (Middle Region)

Catalog No. ABIN360554

Product Details anti-PACSIN1 Antibody

Target: PACSIN1 (Protein Kinase C and Casein Kinase Substrate in Neurons 1 (PACSIN1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PACSIN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to PACSIN1.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human PACSIN1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PACSIN1

Target: PACSIN1 (Protein Kinase C and Casein Kinase Substrate in Neurons 1 (PACSIN1))

Alternative Name: PACSIN1 (PACSIN1 Products)

Synonyms: MGC84055 antibody, sdpii antibody, pacsin1 antibody, x-PACSIN2 antibody, fe25d06 antibody, wu:fe25d06 antibody, zgc:114042 antibody, PACSIN1 antibody, SDPI antibody, A830061D09Rik antibody, H74 antibody, mKIAA1379 antibody, syndapin antibody, protein kinase C and casein kinase substrate in neurons 1 antibody, protein kinase C and casein kinase substrate in neurons 1 S homeolog antibody, protein kinase C and casein kinase substrate in neurons 2 antibody, protein kinase C and casein kinase substrate in neurons 1b antibody, PACSIN1 antibody, pacsin1.S antibody, pacsin2 antibody, pacsin1b antibody, Pacsin1 antibody

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.Synonyms: KIAA1379, Protein kinase C and casein kinase substrate in neurons protein 1

Gene ID: 29993, 9606

UniProt: Q9BY11