Nuclei antibody

Catalog No. ABIN361360

Product Details

Target: Nuclei

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC)

Specificity: The antibody has been directly tested for reactivity in human. This antibody does not react with mouse or rat nuclei. Specific for human nuclei. Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. By immunoprecipitation the antibody reacts with an 80 kDa and 70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.

Cross-Reactivity: Human

Purification: Concentrated tissue culture supernatant

Immunogen: Human cell homogenate.

Clone: 235-1

Isotype: IgG1

Application Details

Application Notes: Recommended Dilution: IF: 1:100 IHC: 1: 100. Quality Control: Immunofluorescence performed on each lot.

Comment:

Normally ABIN361360 should be fix with 10% buffered formalin overnight at room temperature instead of 4% paraformaldehyde. 1.Use 10% buffered formalin overnight instead of 4% paraformaldehyde to fix the tissues 2.Quench the endogenous peroxidase activity with 3% H2O2 instead of 0.5% H2O2 3.Block non-specific binding sites with 3% horse serum instead of goat serum.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: total IgG fraction

Storage: -20 °C

References

Subramanian, Sidhom, Emani, Vernon, Sahakian, Zhou, Kost-Alimova, Slyper, Waldman, Dionne, Nguyen, Weins, Marshall, Rosenblatt-Rosen, Regev, Greka: "Single cell census of human kidney organoids shows reproducibility and diminished off-target cells after transplantation." in: Nature communications, Vol. 10, Issue 1, pp. 5462, (2019) (PubMed).

van den Berg, Ritsma, Avramut, Wiersma, van den Berg, Leuning, Lievers, Koning, Vanslambrouck, Koster, Howden, Takasato, Little, Rabelink: "Renal Subcapsular Transplantation of PSC-Derived Kidney Organoids Induces Neo-vasculogenesis and Significant Glomerular and Tubular Maturation In Vivo." in: Stem cell reports, Vol. 10, Issue 3, pp. 751-765, (2018) (PubMed).

Balseanu, Buga, Catalin, Wagner, Boltze, Zagrean, Reymann, Schaebitz, Popa-Wagner: "Multimodal Approaches for Regenerative Stroke Therapies: Combination of Granulocyte Colony-Stimulating Factor with Bone Marrow Mesenchymal Stem Cells is Not Superior to G-CSF Alone." in: Frontiers in aging neuroscience, Vol. 6, pp. 130, (2014) (PubMed).

Kim, Godarova, Seedle, Kim, Ince, Wells, Driscoll, Godar: "Rb suppresses collective invasion, circulation and metastasis of breast cancer cells in CD44-dependent manner." in: PLoS ONE, Vol. 8, Issue 12, pp. e80590, (2014) (PubMed).

Target Details

Target: Nuclei

Background: This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells.