TUBB3 antibody

Catalog No. ABIN361368

Product Details anti-TUBB3 Antibody

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for the ~55kDa beta III tubulin protein. This clone is similar to the monoclonal antibody Tuj1. It is expected that this antibody will have wide species cross reactivity.

Cross-Reactivity: Rat (Rattus)

Purification: Protein G purified culture supernatant

Immunogen: Synthetic peptide corresponding to amino acid residues specific to beta III tubulin conjugated to KLH

Clone: AA10

Isotype: IgG

Application Details

Application Notes: Recommended Dilution: WB: 1:10,000 IHC: 1:1,000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-TUBB3 antibody (ABIN361368)

Moldovan, Al-Ebraheem, Lobo, Park, Farquharson, Bock: "Altered transition metal homeostasis in the cuprizone model of demyelination." in: Neurotoxicology, Vol. 48, pp. 1-8, (2015) (PubMed).

Stallings, Puttaparthi, Dowling, Luther, Burns, Davis, Elliott: "TDP-43, an ALS linked protein, regulates fat deposition and glucose homeostasis." in: PLoS ONE, Vol. 8, Issue 8, pp. e71793, (2014) (PubMed).

Target Details for TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Alternative Name: TUBB3 (TUBB3 Products)

Synonyms: CDCBM antibody, CFEOM3A antibody, TUBB4 antibody, beta-4 antibody, TUBB3 antibody, 3200002H15Rik antibody, M(beta)3 antibody, M(beta)6 antibody, tubb3 antibody, tubulin beta 3 class III antibody, tubulin, beta 3 class III antibody, tubulin beta 3 class III L homeolog antibody, beta-tubulin 3 antibody, tubulin beta-3 chain antibody, beta tubulin 3 antibody, TUBB3 antibody, Tubb3 antibody, tubb3 antibody, tubb3.L antibody, LOC100581245 antibody, tub3 antibody

Background: Tubulin is the major constituent of microtubules, existing as a heterodimer of the alpha and beta subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins (Lee et al., 1990). Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy (Katsetos et al., 2003) Anti-Beta III Tubulin Western blot of rat cortex lysate showing specific immunolabeling of the ~55k beta III tubulin protein.

Molecular Weight: '55 kDa

Gene ID: 246118

UniProt: Q4QRB4

Pathways: Microtubule Dynamics, M Phase