MARCKS antibody (pSer152, pSer156)

Catalog No. ABIN361478

Product Details anti-MARCKS Antibody

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Binding Specificity: pSer152, pSer156

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCKS antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for the ~87k MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by -phosphatase

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Predicted Reactivity: bovine,chicken, human, Xenopus, zebra fish

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152/156 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Target Details for MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Alternative Name: MARCKS (MARCKS Products)

Synonyms: 80K-L antibody, MACS antibody, PKCSL antibody, PRKCSL antibody, KINC antibody, Macs antibody, marcks antibody, MGC89127 antibody, MARCKS antibody, macs antibody, wu:fb55c06 antibody, wu:fb92d04 antibody, wu:fc19b04 antibody, wu:fc44a10 antibody, zgc:110235 antibody, fc34g03 antibody, fc38c06 antibody, wu:fc34g03 antibody, wu:fc38c06 antibody, zgc:109978 antibody, myristoylated alanine rich protein kinase C substrate antibody, myristoylated alanine-rich protein kinase C substrate antibody, methyl binding domain106 antibody, myristoylated alanine-rich protein kinase C substrate b antibody, myristoylated alanine-rich protein kinase C substrate S homeolog antibody, myristoylated alanine-rich protein kinase C substrate a antibody, MARCKS antibody, Marcks antibody, marcks antibody, mbd106 antibody, marcksb antibody, marcks.S antibody, marcksa antibody

Background: Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al., 1990). The phosphorylation of Ser152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 (Palmer et al., 1996) MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994). Anti-Phospho Ser152/156 MARCKS Western blot of rat brain lysate showing specific immunolabeling of the ~87k MARCKS protein phosphorylated at Serr152,156 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the MARCKS Ser152,156 antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Molecular Weight: '87 kDa

Gene ID: 3028

UniProt: P30009