ALOX5 antibody (pSer523)

Catalog No. ABIN361506

Product Details anti-ALOX5 Antibody

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Binding Specificity: pSer523

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX5 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for the ~80k 5-LO phosphorylated at Ser523 in Western blots.

Cross-Reactivity: Human, Rat (Rattus)

Predicted Reactivity: non human primates

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser523 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Target Details for ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Alternative Name: ALOX5 (ALOX5 Products)

Synonyms: 5-LO antibody, 5-LOX antibody, 5LPG antibody, LOG5 antibody, 5LO antibody, 5LX antibody, AI850497 antibody, F730011J02 antibody, LOX5A antibody, si:dkey-194n13.2 antibody, lox5 antibody, arachidonate 5-lipoxygenase antibody, arachidonate 5-lipoxygenase a antibody, Arachidonate 5-lipoxygenase antibody, ALOX5 antibody, Alox5 antibody, alox5a antibody, AFUA_4G02770 antibody, AOR_1_1362014 antibody, lox5 antibody

Background: The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis. Anti-Phospho Ser523 5-Lipoxygenase Western blot of rat cortex lysate showing specific immunolabeling of the ~80k doublet of 5-LO phosphorylated at Ser523 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the 5-LO Ser523 antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Molecular Weight: '80 kDa

Gene ID: 240

UniProt: P09917