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Mouse anti-Human IgE (Fc Region) Antibody

ELISA, WB, FACS, Func Monoclonal Le27-NBS01 IgG1 kappa unconjugated
Catalog No. ABIN363691
  • Target See all IgE products
    IgE
    Binding Specificity
    • 29
    • 19
    • 17
    • 1
    • 1
    Fc Region
    Reactivity
    • 47
    • 30
    • 10
    • 10
    • 5
    • 4
    • 2
    • 1
    Human
    Host
    • 58
    • 17
    • 10
    • 10
    • 4
    • 1
    Mouse
    Clonality
    • 66
    • 27
    Monoclonal
    Application
    • 54
    • 38
    • 30
    • 20
    • 19
    • 15
    • 12
    • 10
    • 10
    • 8
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
    Specificity
    Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes
    Characteristics
    IGHE, chromosome 14q32.33
    Purification
    Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
    Purity
    > 95 %, tested by analytical Gel Permeation Chromatography
    Immunogen
    purified human myeloma IgE
    Clone
    Le27-NBS01
    Isotype
    IgG1 kappa
  • Application Notes
    For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation is recommended Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb Le27), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    PBS, pH=7.2, 0.1 % NaN3
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
    Storage
    4 °C
  • Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).

  • Target
    IgE
    Abstract
    IgE Products
    Background
    The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.
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