GGA3 antibody (AA 702-713)

Catalog No. ABIN364247

Product Details anti-GGA3 Antibody

Target: GGA3 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3))

Binding Specificity: AA 702-713

Reactivity: Human, Horse, Rabbit, Bat, Monkey, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This GGA3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GGA3. This antibody is expected to recognise both reported isoforms (NP_619525 and NP_054720)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Elephant, Panda, Bat, Horse, Rabbit, Pig (100%) Gibbon, Mouse, Rat, Hamster, Dog, Opossum (92%) Turkey, Chicken, Xenopus (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EQLSTEVGEVDQ from the C-terminus of human GGA3 (NP_619525.1, NP_054720.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Elephant, Panda, Bat, Horse, Rabbit, Pig (100%), Gibbon, Mouse, Rat, Hamster, Dog, Opossum (92%), Turkey, Chicken, Xenopus (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:16000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GGA3

Target: GGA3 (Golgi-Associated, gamma Adaptin Ear Containing, ARF Binding Protein 3 (GGA3))

Alternative Name: GGA3 (GGA3 Products)

Synonyms: C230037M19Rik antibody, mKIAA0154 antibody, golgi associated, gamma adaptin ear containing, ARF binding protein 3 antibody, Gga3 antibody, GGA3 antibody

Background: Name/Gene ID: GGA3

Synonyms: GGA3, KIAA0154

Gene ID: 23163

NCBI Accession: NP_619525, NP_054720

UniProt: Q9NZ52