NDC80 antibody (AA 630-642)

Catalog No. ABIN364285

Product Details anti-NDC80 Antibody

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Binding Specificity: AA 630-642

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NDC80 / HEC1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-YEKKATLIKSSEE from the C-terminus of human NDC80 / HEC1 (NP_006092.1). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: HEC1 / NDC80 (NDC80 Products)

Synonyms: CG9938 antibody, Dmel\\CG9938 antibody, Hec1 antibody, NDC80 antibody, dNdc80 antibody, dmNdc80 antibody, hec1 antibody, KNTC2 antibody, ndc10 antibody, tid3 antibody, 2610020P18Rik antibody, AV139730 antibody, HEC antibody, HEC1 antibody, Kntc2 antibody, kntc2l antibody, wu:fb52e02 antibody, zgc:111801 antibody, HsHec1 antibody, TID3 antibody, hsNDC80 antibody, kntc2 antibody, NDC80, kinetochore complex component antibody, CG9938 gene product from transcript CG9938-RA antibody, spindle pole body protein Ndc80 antibody, kinetochore protein Ndc80 antibody, NDC80 homolog, kinetochore complex component (S. cerevisiae) antibody, NDC80 kinetochore complex component antibody, NDC80 kinetochore complex component S homeolog antibody, NDC80 antibody, Ndc80 antibody, ndc80 antibody, ndc80.S antibody

Background: Name/Gene ID: NDC80

Synonyms: NDC80, HEC1, Kinetochore protein Hec1, Kinetochore associated 2, HEC, HsHec1, HsNDC80, KNTC2, TID3

Gene ID: 10403

NCBI Accession: NP_006092

UniProt: O14777

Pathways: Maintenance of Protein Location