MHC, Class I antibody

Catalog No. ABIN371396

The Mouse Monoclonal anti-MHC, Class I antibody is suitable to detect MHC, Class I in samples from Chicken and Turkey. It has been validated for FACS, WB, IP and IHC (fro).

Quick Overview for MHC, Class I antibody (ABIN371396)

Target: MHC, Class I

Reactivity: Chicken, Turkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC, Class I antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: F21-2

Product Details anti-MHC, Class I Antibody

Characteristics: Synonyms: MHC class I-alpha, Major Histocompatibility Complex Class I

Purification: Purified

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. (Ref.1-4)Immunoprecipitation. (Ref.1-3)Immunohistochemistry on Frozen Sections. Western Blot. Immunoaffinity Chromatography.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for MHC, Class I

Target: MHC, Class I

Alternative Name: MHC Class I

Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (Ref.1-3)Fibroblasts or neurons however only show a low level of antigen.Synonyms: HLA Class 1, MHC Class 1, Major Histocompatibility complex class I