MARCH4 antibody (AA 300-350)

Catalog No. ABIN372267

Product Details anti-MARCH4 Antibody

Target: MARCH4 (Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4))

Binding Specificity: AA 300-350

Reactivity: Human, Mouse, Rat, Dog, Chicken, Horse, Opossum, Zebrafish (Danio rerio), Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody recognizes Membrane-Associated Ring Finger 4 (MARCH4).

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee, Chicken, Dog (Canine), Horse, Mouse, Opossum, Rat and ZebrafishSpecies reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide corresponding to a portion of the amino acids 300-350 of human RNF174.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 5 μg/mL. Western Blot: 0.5-2.0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % Sodium Azide as preservative and 0.05 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for MARCH4

Target: MARCH4 (Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4))

Alternative Name: MARCH4 / RNF174 (MARCH4 Products)

Synonyms: MARCH-IV antibody, RNF174 antibody, BC056494 antibody, mKIAA1399 antibody, MARCH4 antibody, si:ch211-239d11.2 antibody, membrane associated ring-CH-type finger 4 antibody, membrane-associated ring finger (C3HC4) 4 antibody, MARCH4 antibody, March4 antibody, march4 antibody

Background: MARCH4, an E3 ubiquitin protein ligase, may mediate ubiquitination of MHC I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin protein ligases accept ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.Synonyms: E3 ubiquitin-protein ligase MARCH4, KIAA1399, MARCH-IV, Membrane-associated RING finger protein 4, Membrane-associated RING-CH protein IV, RING finger protein 174

Molecular Weight: 46 kDa

Gene ID: 57574

NCBI Accession: NP_065865

UniProt: Q9P2E8