ORAI3 antibody (N-Term)

Catalog No. ABIN372755

Product Details anti-ORAI3 Antibody

Target: ORAI3 (ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORAI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Recognizes ORAI3, also known as Transmembrane Protein 142C, a 31.5 kDa multi-pass membrane protein belonging to the ORAI family. This antibody is not expected to cross react with either ORAI1 or ORAI2.
Cellular Localization: Cell Membrane.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography.

Immunogen: ORAI3 antibody was raised against a 15 amino acid peptide from near the amino terminus of Human ORAI3

Isotype: IgG

Application Details

Application Notes: Western Blot: Detects a band of approximately 35 kDa in A20 cell lysates. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for ORAI3

Target: ORAI3 (ORAI Calcium Release-Activated Calcium Modulator 3 (ORAI3))

Alternative Name: ORAI3 (ORAI3 Products)

Synonyms: ORAI3 antibody, TMEM142C antibody, 9930124N15 antibody, BC061259 antibody, Tmem142c antibody, RGD1306538 antibody, ORAI calcium release-activated calcium modulator 3 antibody, ORAI3 antibody, Orai3 antibody

Background: Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI3 is one of two mammalian homologs of ORAI1, a four- transmembrane spanning protein that is an essential component of CRAC channels. ORAI3, along with ORAI1 and ORAI2, functions as a Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Studies indicate that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2.Synonyms: ORAI-3, TMEM142C, Transmembrane Protein 142C

Molecular Weight: 31 kDa

Gene ID: 93129

NCBI Accession: NP_689501

UniProt: Q9BRQ5