STK35 antibody (C-Term)
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- Target See all STK35 Antibodies
- STK35 (Serine/threonine Kinase 35 (STK35))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat, Dog, Pig, Cow
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This STK35 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificity
- This antibody detects STK35 at C-term.
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse, Rat, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human. - Purification
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography
- Immunogen
- Peptide with sequence ELETRMDQVTCAA corresponding to the C-terminal of Human STK35 (NP_543026.2). Genename: STK35
- Top Product
- Discover our top product STK35 Primary Antibody
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- Application Notes
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Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Approx 45 kDa band observed in human testis lysates (calculatedMW of 44.5 kDa according to NP_543026. Immunohistochemistry: 10 μg/mL. In paraffin embedded human testis shows granularcytoplasmic staining of Leydig cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as presrevtaive and 0.5 % BSA as stabilizer
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- STK35 (Serine/threonine Kinase 35 (STK35))
- Alternative Name
- STK35 (STK35 Products)
- Synonyms
- clik1 antibody, stk35l1 antibody, MGC69346 antibody, im:6905214 antibody, zgc:153287 antibody, MGC115406 antibody, CLIK1 antibody, STK35L1 antibody, 1700054C12Rik antibody, Stk35l1 antibody, serine/threonine kinase 35 antibody, serine/threonine kinase 35 S homeolog antibody, stk35 antibody, stk35.S antibody, STK35 antibody, Stk35 antibody
- Background
- STK35 can interact with PDLIM1/CLP-36 in the cytoplasm and localize to actin stress fibers. This kinase may be a regulator of actin stress fibers in nonmuscle cells. This protein has also been found in the nucleusSynonyms: CLIK-1, CLIK1, CLP-36-interacting kinase 1, PDIK1, PDLIM1-interacting kinase 1, STK35L1, Serine/threonine-protein kinase 35, Serine/threonine-protein kinase 35 L1
- Gene ID
- 140901
- NCBI Accession
- NP_543026
- UniProt
- Q8TDR2
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