B3GNT2 antibody (Internal Region)

Catalog No. ABIN374876

Product Details anti-B3GNT2 Antibody

Target: B3GNT2 (UDP-GlcNAc:BetaGal beta-1,3-N-Acetylglucosaminyltransferase 2 (B3GNT2))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This B3GNT2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-EKHKGFRTFD IE

Specificity: This antibody detects B3GNT2 / B3GALT7.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, RatSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_006568.2. Genename: B3GNT2

Application Details

Application Notes: Peptide ELISA: limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 45 kDa band observed in Human Pancreas lysates(calculated MW of 46.5 kDa according to NP_006568.2). An additional band of unknownidentity was also consistently observed at 25 kDa. This band was successfully blocked byincubation with the immunising peptide. We would appreciate any feedback from peoplein the field - have any such results been reported with other antibodies/lysates? Have anyfurther splice variants/modified forms been reported?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for B3GNT2

Target: B3GNT2 (UDP-GlcNAc:BetaGal beta-1,3-N-Acetylglucosaminyltransferase 2 (B3GNT2))

Abstract: B3GNT2 Products

Synonyms: B3GN-T2 antibody, B3GNT antibody, B3GNT-2 antibody, B3GNT1 antibody, BETA3GNT antibody, BGNT2 antibody, BGnT-2 antibody, AA408337 antibody, AA409404 antibody, AW260308 antibody, B3Galt6 antibody, B3gnt1 antibody, BGnT-1 antibody, BGnT1 antibody, BGnT2 antibody, Beta3gnt antibody, MGC81185 antibody, B3GNT2 antibody, MGC179257 antibody, ssp5 antibody, wu:fa55h04 antibody, wu:fc39c01 antibody, zgc:113947 antibody, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 antibody, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 L homeolog antibody, beta-1,4-glucuronyltransferase 1 antibody, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2b antibody, B3GNT2 antibody, B3gnt2 antibody, b3gnt2.L antibody, B4GAT1 antibody, b3gnt2b antibody, b3gnt2 antibody

Background: B3GNT2 is a member of the beta-1,3-N-acetylglucosaminyltransferase family. It is involved in the initiation and elongation of poly-N-acetyllactosamine chains.Synonyms: 3-GalTase 7, 3-N-acetylglucosaminyltransferase 2, 3-galactosyltransferase 7, B3GNT1, BGnT-1, Beta-1, Beta-1, Beta-3-Gx-T7, Beta3Gal-T7, Beta3Gn-T1, UDP-Gal:beta-GlcNAc beta-1, UDP-GlcNAc:betaGal beta-1, UDP-galactose:beta-N-acetylglucosamine beta-1, b3Gal-T7

Gene ID: 10678, 9606

UniProt: Q9NY97

Pathways: Glycosaminoglycan Metabolic Process