GCH1 antibody (Internal Region)

Catalog No. ABIN375001

Product Details anti-GCH1 Antibody

Target: GCH1 (GTP Cyclohydrolase 1 (GCH1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This GCH1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is expected to recognize all four isoforms.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Peptide with sequence C-GKVHIGYLPNKQ, from the internal region of the protein sequence Genename: GCH1

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GCH1

Target: GCH1 (GTP Cyclohydrolase 1 (GCH1))

Abstract: GCH1 Products

Synonyms: DYT14 antibody, DYT5 antibody, DYT5a antibody, GCH antibody, GTP-CH-1 antibody, GTPCH1 antibody, HPABH4B antibody, GTP-CH antibody, GTPCH antibody, Gch antibody, MGC89622 antibody, gch1a antibody, wu:fc16b10 antibody, zgc:195269 antibody, GTP cyclohydrolase 1 antibody, GTP cyclohydrolase 1-like antibody, GCH1 antibody, Gch1 antibody, gch1 antibody, Weevi_1190 antibody, Halhy_0838 antibody, Sinme_1220 antibody, Sphch_1077 antibody, LOC100136794 antibody

Background: GCH1 is a member of the GTP cyclohydrolase family. It is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described, however, not all variants give rise to a functional enzyme.Synonyms: DYT5, GCH, GTP cyclohydrolase I, GTP-CH-I

Gene ID: 2643, 9606

UniProt: P30793