UBE2F antibody (C-Term)

Catalog No. ABIN375151

Product Details anti-UBE2F Antibody

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2F antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-HLRDKEDFRN KVDD

Specificity: This antibody recognizes NCE2 / UBE2F.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_542409.1. Genename: UBE2F

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.05 - 0.2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Alternative Name: NCE2 (UBE2F Products)

Synonyms: NCE2 antibody, 2510010F15Rik antibody, AI851109 antibody, RGD1307608 antibody, zgc:77005 antibody, ubiquitin conjugating enzyme E2 F (putative) antibody, NEDD8-conjugating enzyme UBE2F antibody, ubiquitin-conjugating enzyme E2F (putative) antibody, ubiquitin conjugating enzyme E2F (putative) S homeolog antibody, UBE2F antibody, Tsp_01616 antibody, ube2f antibody, ube2f.S antibody, Ube2f antibody

Background: NCE2 is a ubiquitin conjugating enzyme. Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.Synonyms: NEDD8-conjugating enzyme 2, UBE2F, Ubiquitin-conjugating enzyme E2 F

Molecular Weight: 21.1kDa (Human NP_542409.1 and Mouse NP_080730.1).

Gene ID: 140739, 9606

UniProt: Q969M7