Ly-6G/Ly-6C antibody (PE-Cy7)

Details for Product No. ABIN375387
Antigen
Reactivity
Mouse (Murine)
65
Host
Rat
65
Clonality (Clone)
Monoclonal ()
Conjugate
PE-Cy7
10
9
6
6
5
5
3
2
2
2
2
2
1
1
1
1
Application
Flow Cytometry (FACS)
65
4
4
4
4
4
4
1
Options
Immunogen Unknown
Clone RB6-8C5
Isotype IgG2b
Specificity Mouse Ly-6G/Ly-6C, Mr 21-25 kDa
Characteristics Rat Anti-Mouse Ly-6G/Ly-6C-PE/CY7
Purification Purified
Antigen
Background Ly-6G, formerly known as the myeloid differentiation antigen Gr-1, is a GPI-anchored protein. 1 In the bone marrow, the level of antigen expression is directly correlated with granulocyte differentiation and maturation. It is also transiently expressed on monocytes in the bone marrow. 2 In the periphery, Ly-6G is expressed predominantly on neutrophils.
Application Notes
  • Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , CMCD - Reported in literature , Depletion - Reported in literature
  • Working Dilutions: Flow Cytometry FITC, BIOT, AF488, and PACBLU conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells SPRD, APC, CY5, PE/CY5.5, PE/CY7, APC/CY7, and AF700 0.03 g/106 cells conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Comment

In vitro complement-dependent cytotoxicity assays, In vivo depletion of Ly-6G+ cells

Sample Volume 1 mL
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer 0.1 mg in 0.2 mL of PBS/Sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Storage Comment Store at 2-8°C
Supplier Images
 image for anti-Ly-6G/Ly-6C antibody (PE-Cy7) (ABIN375387) BALB/c mouse bone marrow cells were stained with Rat Anti-Mouse Ly-6G/Ly-6C-PE/CY7.
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