CD33 antibody (APC)

Catalog No. ABIN375819

Product Details anti-CD33 Antibody (APC)

Target: CD33

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD33 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: Human CD33, Mr 67 kDa

Characteristics: Mouse Anti-Human CD33-APC

Purification: Purified

Immunogen: Unknown

Clone: WM53

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , WB - Reported in literature , Block - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, PE, APC, SPRD, PE/CY5.5, PACBLU, AF488, AF647, and 10 L/106 cells AF700 conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Buffer: 25 tests in 0.25 mL or 100 tests in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for CD33

Target: CD33

Alternative Name: CD33 (CD33 Products)

Background: CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function.