Goat anti-Mouse Ig (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN376237

Product Details

Target: Ig

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Reacts with the heavy and light chains of mouse IgM, IgG 1, IgG 2a, IgG 2b, IgG 3 and IgA as demonstrated by ELISA and flow cytometry.

Characteristics: Source: Pooled antisera from goats hyperimmunized with mouse IgM, IgG and IgA paraproteins.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry to conform to characteristics of a standard reference reagent.

Purification: Affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose.

Isotype: IgG

Application Details

Application Notes: Working Dilution:
1:4,000-1:8,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

References

Zantow, Just, Lagkouvardos, Kisling, Dübel, Lepage, Clavel, Hust: "Mining gut microbiome oligopeptides by functional metaproteome display." in: Scientific reports, Vol. 6, pp. 34337, (2016) (PubMed).

Target Details

Target: Ig

Abstract: Ig Products