Goat anti-Mouse IgA (Heavy Chain) Antibody (HRP)

Catalog No. ABIN376245

Product Details

Target: IgA

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Reacts with the heavy chain of mouse IgA as demonstrated by ELISA and flow cytometry.

Characteristics: Source: Pooled antisera from goats hyperimmunized with mouse IgA paraproteins.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance with characteristics of a standard reference reagent.

Purification: Affinity chromatography on pooled mouse Igs with λ light chains covalently linked to agarose.

Isotype: IgG

Application Details

Application Notes: Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

References

Abhyankar, Orr, Lin, Suraju, Simpson, Blust, Pham, Guderian, Tomai, Elvecrog, Pedersen, Petri, Fox: "Adjuvant composition and delivery route shape immune response quality and protective efficacy of a recombinant vaccine for Entamoeba histolytica." in: NPJ vaccines, Vol. 3, pp. 22, (2018) (PubMed).

Tang, Shang, Chen, Ren, Sun, Qu, Lin, Zhou, Yu, Jiang, Zhou, Li, Huang, Xu, Yu: "The immunological characteristics and probiotic function of recombinant Bacillus subtilis spore expressing Clonorchis sinensis cysteine protease." in: Parasites & vectors, Vol. 9, Issue 1, pp. 648, (2017) (PubMed).

Sawaengsak, Mori, Yamanishi, Srimanote, Chaicumpa, Mitrevej, Sinchaipanid: "Intranasal chitosan-DNA vaccines that protect across influenza virus subtypes." in: International journal of pharmaceutics, Vol. 473, Issue 1-2, pp. 113-125, (2014) (PubMed).

Sanjo, Hikida, Aiba, Mori, Hatano, Ogata, Kurosaki: "Extracellular signal-regulated protein kinase 2 is required for efficient generation of B cells bearing antigen-specific immunoglobulin G." in: Molecular and cellular biology, Vol. 27, Issue 4, pp. 1236-46, (2007) (PubMed).

Rice, Peng, Spence, Wang, Goldblum, Corthésy, Nowicki: "Pyelonephritic Escherichia coli expressing P fimbriae decrease immune response of the mouse kidney." in: Journal of the American Society of Nephrology : JASN, Vol. 16, Issue 12, pp. 3583-91, (2006) (PubMed).

Dunn, Upcroft, Fowler, Matthews, Upcroft: "Orally administered Giardia duodenalis extracts enhance an antigen-specific antibody response." in: Infection and immunity, Vol. 69, Issue 10, pp. 6503-10, (2001) (PubMed).

Target Details

Target: IgA

Abstract: IgA Products

Target Type: Antibody