Goat anti-Human IgG Antibody (PE) - Preadsorbed

Catalog No. ABIN376512

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: PE

Application: ELISA, Flow Cytometry (FACS)

Specificity: Reacts with the heavy chain of human IgG

Characteristics: Goat F(ab')2 Anti-Human IgG, Mouse ads-PE

Purification:

Preadsorption: Mouse adsorbed

Isotype: IgG

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA FLISA FC
• Working Dilutions: ELISA BIOT conjugate 1:5,000 - 1:20,000 HRP conjugate 1:4,000 - 1:8,000 FLISA FITC and AF555 conjugates 1:100 - 1:400 PE conjugate 1 g/mL Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Buffer: 0.25 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Brannan, He, Howell, Prugar, Zhu, Vu, Shulenin, Kailasan, Raina, Wong, Rahim, Banadyga, Tierney, Zhao, Li, Holtsberg, Dye, Qiu, Aman: "Post-exposure immunotherapy for two ebolaviruses and Marburg virus in nonhuman primates." in: Nature communications, Vol. 10, Issue 1, pp. 105, (2019) (PubMed).

Gallery, Zhang, Bradley, Brauer, Cvet, Estevam, Danaee, Greenfield, Li, Manfredi, Loke, Rabino, Stringer, Williamson, Wyant, Yang, Zhu, Abu-Yousif, Veiby: "A monomethyl auristatin E-conjugated antibody to guanylyl cyclase C is cytotoxic to target-expressing cells in vitro and in vivo." in: PLoS ONE, Vol. 13, Issue 1, pp. e0191046, (2018) (PubMed).

Kristian, Ota, Bubeck, Cho, Groff, Kubota, Destito, Martin, Laudenslager, Koriazova, Tahara, Kanda: "Generation and Improvement of Effector Function of a Novel Broadly Reactive and Protective Monoclonal Antibody against Pneumococcal Surface Protein A of Streptococcus pneumoniae." in: PLoS ONE, Vol. 11, Issue 5, pp. e0154616, (2017) (PubMed).

Schubert, Bode, Kenefeck, Hou, Wing, Kennedy, Bulashevska, Petersen, Schäffer, Grüning, Unger, Frede, Baumann, Witte, Schmidt, Dueckers, Niehues, Seneviratne, Kanariou, Speckmann, Ehl, Rensing-Ehl et al.: "Autosomal dominant immune dysregulation syndrome in humans with CTLA4 mutations. ..." in: Nature medicine, Vol. 20, Issue 12, pp. 1410-1416, (2015) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody