FADD antibody (Fas (TNFRSF6)-Associated Via Death Domain)

Details for Product anti-FADD Antibody No. ABIN377014
Antigen
Reactivity
Human
204
103
42
9
1
Host
Mouse
221
47
Clonality (Clone)
Monoclonal ()
Conjugate
This FADD antibody is un-conjugated
11
7
6
6
6
6
6
6
5
4
4
4
3
1
1
1
1
1
1
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
219
117
83
36
20
16
15
9
8
4
4
2
1
Options
Immunogen Recombinant FADD
Clone 1A11
Isotype IgG1
Specificity Human FADD
Characteristics Mouse Anti-Human FADD-UNLB
Purification Purified
Antigen
Alternative Name FADD (FADD Antibody Abstract)
Background Fas-associated death domain (FADD, also known as MORT1) is an important apoptosis adapter molecule involved in the Fas-mediated cell death pathway. FADD associates with the intracellular domain of FAS (CD95/APO-1) through their respective C-terminal death domains (DD). The N-terminal region of FADD contains a death effectors domain (DED) which recruits and binds cytosolic proteins such as FLICE/caspase-8. The subsequent activation of FLICE by proteolytic cleavage leads to the activation of the caspase-mediated (ICE-related proteases) apoptotic cascade. Overexpression of FADD induces apoptosis which is inhibited by CrmA, a specific inhibitor of the interleukin-1 β -converting enzyme. FADD has also been shown under certain circumstances to promote cell survival and proliferation.
Pathways Apoptosis, TLR Signaling, Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
Application Notes
  • Applications: ELISA - Quality tested WB - Quality tested IP - Quality tested
  • Working Dilutions: ELISA Purified (UNLB) antibody 0.5 g/mL Immunoblotting HRP conjugate 1:4,000 - 1:8,000 Immunoprecipitation SEPH conjugate 40 L
Sample Volume 0,2 mL
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer 0.1 mg of purified immunoglobulin in 0.2 mL of borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added
Preservative Without preservative
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Storage Comment Store at 2-8°C
Supplier Images
Image no. 1 for anti-Fas (TNFRSF6)-Associated Via Death Domain (FADD) antibody (ABIN377014) Total cell lysates from Jurkat cells were resolved by electrophoresis, transferred to...