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beta Actin antibody

ACTB Reactivity: Human, Mouse, Chicken, Rabbit, Pig, Cow, Hamster, Goat, Fish WB, ELISA Host: Mouse Monoclonal 2D1D10 unconjugated
Catalog No. ABIN387703
  • Target See all beta Actin (ACTB) Antibodies
    beta Actin (ACTB) (Actin, beta (ACTB))
    Reactivity
    • 192
    • 133
    • 120
    • 64
    • 51
    • 44
    • 37
    • 36
    • 32
    • 25
    • 20
    • 20
    • 12
    • 10
    • 9
    • 8
    • 7
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Chicken, Rabbit, Pig, Cow, Hamster, Goat, Fish
    Host
    • 116
    • 93
    • 5
    • 2
    • 1
    Mouse
    Clonality
    • 110
    • 105
    Monoclonal
    Conjugate
    • 134
    • 15
    • 14
    • 10
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This beta Actin antibody is un-conjugated
    Application
    • 182
    • 76
    • 58
    • 54
    • 37
    • 36
    • 28
    • 24
    • 13
    • 13
    • 7
    • 6
    • 6
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Specificity
    THETM beta Actin Antibody, mAb, Mouse reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig. It has not yet been tested in other species.
    Cross-Reactivity (Details)
    Cross reactivity in other species has not yet been tested.
    Purification
    Protein G affinity column
    Immunogen
    A synthetic peptide DDDIAALVVDNGSG coupled - KLH
    Clone
    2D1D10
    Isotype
    IgG
    Top Product
    Discover our top product ACTB Primary Antibody
  • Application Notes
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.1-1.0 µg/mL
    Western blot: 1.0 µg/mL
    Other applications: user-optimized
    Restrictions
    For Research Use only
  • Validation #102832 (Western Blotting)
    'Independent Validation' Badge
    by
    Dr. Randy Brutkiewicz Laboratory, Department of Microbiology and Immunology, Indiana University School of Medicine
    No.
    #102832
    Date
    02/20/2018
    Antigen
    ACTB
    Lot Number
    Method validated
    Western Blotting
    Positive Control
    lysates from HEK293 cells untransfected or transfected with human MR1 cDNA
    Negative Control
    Notes

    Passed. ABIN962807 recognizes human beta actin in HEK293 cell lysates.

    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN962807
    Secondary Antibody
    goat anti-rabbit Dye-IR800 conjugated antibody (Advansta, R-05060-250, lot 17083179)
    Full Protocol
    • Grow HEK293 cells in DMEM medium (Lonza, 12-614F, lot 0000618582) supplemented with serum (Hyclone, SH30071.03, lot AAG205460) and antibiotics (Hyclone, SV30010, lot J150013), at 37°C and 5% CO2 dish to 70-90% confluency.
    • Transfect cells with pCDNA 3.1 neo (-) (Invitrogen) containing human MR1 cDNA (Genecopoeia) using Polyethylenimine (Polysciences, 23966) following the manufacturer´s instructions.
    • Lyse cells in cold lysis buffer (10mM Tris pH7.4, 150mM NaCl, 0.5mM EDTA, 2% CHAPS).
    • Determine total protein content of the lysates using Commassie Protein Assay Reagent (Thermo Scientific, 1856209, lot NL179252).
    • Denature 200µg total protein for 5min at 95°C in 20µl Laemmli SDS sample buffer and subsequently separate them on a SDS-PAGE gel using Acrylamide/Bis Premixed (Bio-Rad, 61-0125, lot 260000477) for 2-3h at 100V.
    • Transfer proteins onto PVDF membrane (Millipore, IPVH00010, lot K5AA6843U) with a Western blotting system for ON at 4°C at 150mA.
    • Block the membrane with blocking buffer (2% BSA/PBS/0.05%Tween-20) for 1h at RT.
    • Incubate membrane with:
      • loading control rabbit anti beta-actin (antibodies-online, ABIN962807) diluted 1:500 in blocking buffer ON at 4°C.
      • primary rabbit anti-MR1 antibody (antibodies-online, ABIN1537116, lot SA111213CH) diluted 1:1000 in blocking buffer ON at 4°C.
    • Wash membrane 3x for 10min with PBS/0.05%Tween-20.
    • Incubate membrane with secondary goat anti-rabbit Dye-IR800 conjugated antibody (Advansta, R-05060-250, lot 17083179) diluted 1:10000 in PBS/0.05% Tween-20 for 1h at RT.
    • Wash membrane 3x for 10min with PBS/0.05% Tween-20.
    • Reveal protein bands using an Odyssey imaging system (LI-COR Biosciences).
    Experimental Notes

    The beta Actin antibody ABIN962807 reveals a protein of the expected molecular weight of beta actin in lysates of human HEK293 cells.

  • Format
    Lyophilized
    Reconstitution
    Reconstitute the lyophilized antibody with deionized water to the final concentration of 0.5 mg/mL.
    Buffer
    PBS, pH 7.4, containing 0.02 % Sodium azide, without BSA
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Storage
    4 °C/-20 °C
    Storage Comment
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Zhao, Mo, Li, Zou, Cheng, Sun, Xiong, Guan, Lei: "NOTCH-induced aldehyde dehydrogenase 1A1 deacetylation promotes breast cancer stem cells." in: The Journal of clinical investigation, (2014) (PubMed).

    Srivastava, Reinoso, Singh, Fernandez-Bueno, Martino, Garcia-Gutierrez, Pastor, Corell: "Flow cytometry assessment of the purity of human retinal pigment epithelial primary cell cultures." in: Journal of immunological methods, Vol. 389, Issue 1-2, pp. 61-8, (2013) (PubMed).

  • Target
    beta Actin (ACTB) (Actin, beta (ACTB))
    Alternative Name
    beta Actin (ACTB Products)
    Synonyms
    BRWS1 antibody, PS1TP5BP1 antibody, ACTA1 antibody, Actx antibody, E430023M04Rik antibody, beta-actin antibody, ps1tp5bp1 antibody, act-2 antibody, actb antibody, B-actin antibody, LOC100174883 antibody, LOC100304816 antibody, A antibody, ACT5C antibody, Ac5C antibody, Act antibody, Act-5C antibody, Act5 antibody, Act5c antibody, Actin antibody, Actin/BAP47 antibody, Actin5C antibody, BAP47 antibody, Bap47 antibody, CG4027 antibody, Dmel\\CG4027 antibody, M32055 antibody, T11 antibody, act antibody, act 5C antibody, act5C antibody, actin antibody, actin5C antibody, anon-EST:fe2D2 antibody, beta-actin/Bap47 antibody, cyt5C antibody, l(1)G0009 antibody, l(1)G0010 antibody, l(1)G0025 antibody, l(1)G0079 antibody, l(1)G0117 antibody, l(1)G0177 antibody, l(1)G0245 antibody, l(1)G0330 antibody, l(1)G0420 antibody, l(1)G0486 antibody, Bact antibody, ACT-5 antibody, ACTB antibody, B-ACTZF antibody, actba antibody, bact antibody, bactin1 antibody, bactzf antibody, wu:fd18f05 antibody, A26C1B antibody, POTE2alpha antibody, POTEACTIN antibody, Beta-actin antibody, actin beta antibody, actin, beta antibody, cytoplasmic actin OlCA1 antibody, beta-actin antibody, Actin 5C antibody, actin gamma 1 antibody, actin, beta 1 antibody, actin, beta L homeolog antibody, beta actin antibody, POTE ankyrin domain family member F antibody, actin, beta like 2 antibody, actin, cytoplasmic 1 antibody, ACTB antibody, Actb antibody, actb antibody, LOC100174883 antibody, LOC100304816 antibody, Act5C antibody, ACTG1 antibody, actb1 antibody, actb.L antibody, LOC100136352 antibody, POTEF antibody, ACTBL2 antibody, LOC109073280 antibody
    Background
    Beta-actin is one of the six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. As a consequence, it has been difficult to produce potent antisera against this protein. Therefore, the availability of monoclonal antibodies to beta-actin provides a useful and specific tool in the study of the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
    Pathways
    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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