ZMIZ1 antibody (C-Term)

Catalog No. ABIN390174

Product Details anti-ZMIZ1 Antibody

Target: ZMIZ1 (Zinc Finger, MIZ-Type Containing 1 (ZMIZ1))

Binding Specificity: AA 1038-1067, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZMIZ1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: M

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This RAI17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1038-1067 amino acids from the C-terminal region of human RAI17.

Clone: RB1963

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-ZMIZ1 antibody (ABIN390174)

Lan, Lan, Chang, Zhang, Liu, Vikash, Wang, Huang, Wang, Zhou, Chen, Zhao: "Identification of Two Additional Susceptibility Loci for Inflammatory Bowel Disease in a Chinese Population." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 41, Issue 5, pp. 2077-2090, (2017) (PubMed).

Pinnell, Yan, Cho, Keeley, Murai, Liu, Alarcon, Qin, Wang, Kuick, Elenitoba-Johnson, Maillard, Samuelson, Cierpicki, Chiang: "The PIAS-like Coactivator Zmiz1 Is a Direct and Selective Cofactor of Notch1 in T Cell Development and Leukemia." in: Immunity, Vol. 43, Issue 5, pp. 870-83, (2015) (PubMed).

Rakowski, Garagiola, Li, Decker, Caruso, Jones, Kuick, Cierpicki, Maillard, Chiang: "Convergence of the ZMIZ1 and NOTCH1 pathways at C-MYC in acute T lymphoblastic leukemias." in: Cancer research, Vol. 73, Issue 2, pp. 930-41, (2013) (PubMed).

Target Details for ZMIZ1

Target: ZMIZ1 (Zinc Finger, MIZ-Type Containing 1 (ZMIZ1))

Alternative Name: RAI17 (ZMIZ1 Products)

Synonyms: miz antibody, rai17 antibody, zimz1 antibody, zimp10 antibody, hzimp10 antibody, MIZ antibody, RAI17 antibody, TRAFIP10 antibody, ZIMP10 antibody, hZIMP10 antibody, BC065120 antibody, E330020C23 antibody, ENSMUSG00000072684 antibody, Gm10397 antibody, I920194n01 antibody, Rai17 antibody, Zimp10 antibody, wu:fb80e10 antibody, zgc:175159 antibody, zmiz1 antibody, zinc finger MIZ-type containing 1 antibody, zinc finger, MIZ-type containing 1 antibody, zinc finger, MIZ-type containing 1a antibody, ZMIZ1 antibody, zmiz1 antibody, Zmiz1 antibody, zmiz1a antibody

Background: Retinoic acid plays a critical role in development, cellular growth, and differentiation and induces the expression of a variety of genes. RAI17 expression is induced by retinoic acid and is predominantly expressed in heart, brain and ovaries. Within brain, highest expression is in amygdala. The deduced 1,067-amino acid protein contains an MSX-interacting zinc finger (MIZ) domain, a nuclear localization signal sequence, and 2 proline-rich regions. A strong intrinsic transactivation domain is identified in the C-terminal proline-rich region. RAI17 expression is detected in various cancer cell lines. RAI17 colocalizes with endogenous androgen receptor (AR) in the nuclei of prostate epithelial cells from human tissue samples. In human prostate cancer cells, RAI17 increases the transcriptional activity of AR. Studies using sumoylation-deficient AR mutants suggest that the increase of AR activity by RAI17 is dependent upon receptor sumoylation.

Molecular Weight: 115483

Gene ID: 57178

NCBI Accession: NP_065071

UniProt: Q9ULJ6