DAGLA antibody (AA 1031-1042)

Catalog No. ABIN396920

Product Details anti-DAGLA Antibody

Target: DAGLA (Diacylglycerol Lipase, alpha (DAGLA))

Binding Specificity: AA 1031-1042

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DAGLA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human DAGLA.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Elephant, Panda (92%) Mouse, Rat, Hamster, Bovine, Rabbit, Horse, Pig (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-PAKQDELVISAR from the C-terminus of human DAGLA (NP_006124.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Elephant, Panda (92%), Mouse, Rat, Hamster, Bovine, Rabbit, Horse, Pig (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000, 0.3 - 1 μg/mL), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 125kD band observed in Human Liver lysates (calculated MW of 115kD according to NP_006124.1). Recommended concentration: 0.3-1 μg/mL. Some minor background is detected and is blocked by the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for DAGLA

Target: DAGLA (Diacylglycerol Lipase, alpha (DAGLA))

Alternative Name: DAGLA (DAGLA Products)

Synonyms: C11orf11 antibody, DAGL(ALPHA) antibody, DAGLALPHA antibody, NSDDR antibody, Nsddr antibody, diacylglycerol lipase alpha antibody, diacylglycerol lipase, alpha antibody, DAGLA antibody, dagla antibody, Dagla antibody

Background: Name/Gene ID: DAGLA

Synonyms: DAGLA, DAGLALPHA, DGL-alpha, Diacylglycerol lipase, alpha, KIAA0659, C11orf11, DAGL(ALPHA), NSDDR

Gene ID: 747

NCBI Accession: NP_006124

UniProt: Q9Y4D2