HRP antibody

Catalog No. ABIN398438

Product Details anti-HRP Antibody

Target: HRP (Horseradish Peroxidase (HRP))

Reactivity: Horseradish

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRP antibody is un-conjugated

Application: ELISA

Specificity: Rabbit Anti-HRP Polyclonal Antibody is specific to peroxidase (horseradish).

Purification: Protein G chromatography

Immunogen: Purified peroxidase from horseradish

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.5 mg/mL

Buffer: PBS, pH 7.4, containing 30 % glycerol, and 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-HRP antibody (ABIN398438)

Du, Chen, Zhan, Mu, Ren, Zuo: "A new insight to explore the regulation between S-nitrosylation and N-glycosylation." in: Plant direct, Vol. 3, Issue 2, pp. e00110, (2019) (PubMed).

Radhakrishnan, Origenes, Littlejohn, Nikolich, Choi, Smite, Lamoureux, Baetiong, Shah, Gazmuri: "Plasma Cytochrome c Detection Using a Highly Sensitive Electrochemiluminescence Enzyme-Linked Immunosorbent Assay." in: Biomarker insights, Vol. 12, pp. 1177271917746972, (2017) (PubMed).

Wiersma, de Bruyn, Shi, Gooden, Wouters, Samplonius, Hendriks, Nijman, Wei, Zhou, Helfrich, Bremer: "C-type lectin-like molecule-1 (CLL1)-targeted TRAIL augments the tumoricidal activity of granulocytes and potentiates therapeutic antibody-dependent cell-mediated cytotoxicity." in: mAbs, Vol. 7, Issue 2, pp. 321-30, (2015) (PubMed).

Wang, Xu, Li, Zhang, Lim, Lee, Li, Qian, Jiang, Qi: "OsMOGS is required for N-glycan formation and auxin-mediated root development in rice (Oryza sativa L.)." in: The Plant journal : for cell and molecular biology, Vol. 78, Issue 4, pp. 632-45, (2014) (PubMed).

Zhang, Li, Jiang, Chen: "Use of MSG1 protein in a novel blocking ELISA for the detection of Mycoplasma suis infection." in: Veterinary journal (London, England : 1997), Vol. 193, Issue 2, pp. 535-8, (2012) (PubMed).

Fernandez-Funez, Zhang, Casas-Tinto, Xiao, Zou, Rincon-Limas: "Sequence-dependent prion protein misfolding and neurotoxicity." in: The Journal of biological chemistry, Vol. 285, Issue 47, pp. 36897-908, (2010) (PubMed).

Target Details for HRP

Target: HRP (Horseradish Peroxidase (HRP))

Alternative Name: HRP (HRP Products)

Background: Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator enzyme in reactions in which peroxide is produced, such as in conjunction with glucose oxidase in the evaluation of glucose in biological fluids. It also can be used as an enzyme label, such as in ELISA systems and peroxidase-anti-peroxidase complexes in immunohistochemistry. Rabbit Anti-HRP Polyclonal Antibody is developed in rabbit hosts using purified peroxidase from horseradish as the immunogen.Rabbit Anti-HRP Polyclonal Antibody is purified from rabbit antiserum by Protein G chromatography and is supplied as a 200 µg aliquot at a concentration of 0.5 mg/ml.