GST antibody

Catalog No. ABIN398843

Product Details anti-GST Antibody

Target: GST (Glutathione S Transferase (GST))

Reactivity: Schistosoma japonicum

Host: Goat

Clonality: Polyclonal

Conjugate: This GST antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Goat Anti-GST-tag Polyclonal Antibody detects GST fusion proteins in almost expression systems including bacterial expression system, mammalian expression system and other systems.

Purification: Immunoaffinity chromatography

Immunogen: Recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum.

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.2-1 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-GST antibody (ABIN398843)

Lichtenstein, Targan, Dubinsky, Rotter, Barken, Princen, Carroll, Brown, Stachelski, Chuang, Landers, Stempak, Singh, Silverberg: "Combination of genetic and quantitative serological immune markers are associated with complicated Crohn's disease behavior." in: Inflammatory bowel diseases, Vol. 17, Issue 12, pp. 2488-96, (2011) (PubMed).

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: Glutathione-S-Transferase (GST) (GST Products)

Synonyms: GST antibody, GST 13-13 antibody, GST13 antibody, GST13-13 antibody, GSTK1-1 antibody, hGSTK1 antibody, 1500002K10Rik antibody, Gst antibody, Ptgds2 antibody, glutathione S-transferase kappa 1 antibody, microsomal glutathione S-transferase 1 antibody, hematopoietic prostaglandin D synthase antibody, GSTK1 antibody, Mgst1 antibody, Hpgds antibody

Background: Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogeneity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be used in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.Goat Anti-GST-tag Polyclonal Antibody is developed in goat using a recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum.