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GST antibody (HRP)

GST Reactivity: Schistosoma japonicum WB, ELISA Host: Goat Polyclonal HRP
Catalog No. ABIN398845
  • Target See all GST Antibodies
    GST (Glutathione S Transferase (GST))
    Reactivity
    • 76
    • 33
    • 31
    • 11
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Schistosoma japonicum
    Host
    • 62
    • 59
    • 43
    • 2
    • 2
    • 1
    Goat
    Clonality
    • 102
    • 65
    Polyclonal
    Conjugate
    • 74
    • 13
    • 11
    • 10
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GST antibody is conjugated to HRP
    Application
    • 141
    • 75
    • 36
    • 29
    • 22
    • 17
    • 16
    • 14
    • 10
    • 8
    • 8
    • 7
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Specificity
    Goat Anti-GST-tag [HRP] Polyclonal Antibody detects GST fusion proteins in expression systems including bacterial expression system, mammalian expression system and others.
    Purification
    Immunoaffinity chromatography
    Immunogen
    Recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum.
    Isotype
    IgG
    Top Product
    Discover our top product GST Primary Antibody
  • Application Notes
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0.05-0.2 µg/mL Western blot: 0.2-1 µg/mLOther Applications: user-optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
    Buffer
    PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal.
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Savar, Jahanian-Najafabadi, Mahdavi, Shokrgozar, Jafari, Bouzari et al.: "In silico and in vivo studies of truncated forms of flagellin (FliC) of enteroaggregative Escherichia coli fused to FimH from uropathogenic Escherichia coli as a vaccine candidate against urinary ..." in: Journal of biotechnology, Vol. 175, pp. 31-7, (2014) (PubMed).

    Gish, Gagne, Han, Deyoung, Clark: "WUSCHEL-responsive At5g65480 interacts with CLAVATA components in vitro and in transient expression." in: PLoS ONE, Vol. 8, Issue 6, pp. e66345, (2013) (PubMed).

  • Target
    GST (Glutathione S Transferase (GST))
    Alternative Name
    Glutathione-S-Transferase (GST) (GST Products)
    Synonyms
    GST antibody, GST 13-13 antibody, GST13 antibody, GST13-13 antibody, GSTK1-1 antibody, hGSTK1 antibody, 1500002K10Rik antibody, Gst antibody, Ptgds2 antibody, glutathione S-transferase kappa 1 antibody, microsomal glutathione S-transferase 1 antibody, hematopoietic prostaglandin D synthase antibody, GSTK1 antibody, Mgst1 antibody, Hpgds antibody
    Background
    Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogeneity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished by using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by introducing site-specific proteases. Antibodies directed against GST can detect both the fusion protein during purification and the cleavage of GST from the protein of interest.Goat Anti-GST-tag [HRP] Polyclonal Antibody is developed in goat using a recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum. The antibody is highly purified by immunoaffinity chromatography from goat antiserum and is then conjugated to horseradish peroxidase.
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