CD56 antibody (AA 780-830)

Catalog No. ABIN398892

Product Details anti-CD56 Antibody

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Binding Specificity: AA 780-830

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD56 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Rabbit Anti-NCAM Polyclonal Antibody detects endogenous levels of rat and mouse NCAM. It is predicted to react with human NCAM according to sequence homology.

Cross-Reactivity (Details): Rabbit Anti-NCAM Polyclonal Antibody detects endogenous levels of rat and mouse NCAM. It is predicted to react with human NCAM according to sequence homology.

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide within cytoplasmic AA 780-830 of human NCAM .

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Alternative Name: NCAM (NCAM1 Products)

Synonyms: CD56 antibody, MSK39 antibody, NCAM antibody, fj56g09 antibody, ncam antibody, ncam1 antibody, wu:fj56g09 antibody, NCAM1 antibody, n-cam antibody, E-NCAM antibody, NCAM-1 antibody, Ncam antibody, Cd56 antibody, N-CAM antibody, N-CAM-1 antibody, NCAM-C antibody, NCAMC antibody, N-CAM-1-B antibody, NCAM-1-B antibody, ncam1-a antibody, ncam1-b antibody, neural cell adhesion molecule 1 antibody, neural cell adhesion molecule 1a antibody, neural cell adhesion molecule 1 S homeolog antibody, neural cell adhesion molecule 1 L homeolog antibody, NCAM1 antibody, ncam1a antibody, ncam1 antibody, LOC100085373 antibody, ncam1.S antibody, Ncam1 antibody, ncam1.L antibody

Background: NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein. It contains five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. Alternative splicing gives rise to different cytoplasmic domains. NCAM is involved in neuronal attachment, neurite extension, and cell-cell interactions through homo and heterophilic interactions. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. It is expressed on most neuroectodermal derived cell lines, tissues, and neoplasm.Rabbit Anti-NCAM Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within cytoplasmic residues 780-830 of human NCAM (Swiss Prot: P13591).