IL-13 antibody

Catalog No. ABIN401064

Product Details anti-IL-13 Antibody

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-13 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to Interleukin-13.

Cross-Reactivity (Details): Species reactivity (expected):Sheep, Dolphin, Beluga, Bovine, Human, Certain monkey, Dusky titi, Macaque, Baboon, Mangabey, Canine, Llama, Camel, Galago, Horse, Rat, Mouse, Gerbil.
Species reactivity (tested):Porcine.

Purification: Affinity chromatography on Protein A

Immunogen: Full length recombinant protein raised in yeast, corresponding to mature swine IL-13 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/11,000. Western Blot: 1.5 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 100 μL of deionized water or equivalent.

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: Interleukin-13 / IL13 (IL13 Products)

Synonyms: IL13 antibody, IL-13 antibody, P600 antibody, Il-13 antibody, L13 antibody, interleukin 13 antibody, IL13 antibody, Il13 antibody

Background: Interleukin 13 (IL-13), of the IL-4 superfamily, is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Ra) and at least one IL-13-specific binding chain. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, STAT6. In humans, IL-13 can induce immunoglobulin E (IgE) secretion from activated B cells. In mice, deletion of IL-13 does not markedly affect either Th2 cell development or antigen-specific IgE responses induced by potent allergens. Deletion of IL-4 abrogates these responses. IL-13 acts as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thus altering physiological function. Although IL-13 is associated primarily with the induction of airway disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, it also induces airway matrix metalloproteinases as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation.Synonyms: IL-13, NC30

Gene ID: 396721

NCBI Accession: NP_998968

UniProt: Q95J68

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport