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RFP antibody (Biotin)

RFP Reactivity: Discosoma WB, ELISA, IHC (fro) Host: Rabbit Polyclonal Biotin
Catalog No. ABIN401226
  • Target See all RFP Antibodies
    RFP (Red Fluorescent Protein (RFP))
    Reactivity
    Discosoma
    Host
    • 23
    • 13
    • 9
    • 5
    • 3
    Rabbit
    Clonality
    • 30
    • 23
    Polyclonal
    Conjugate
    • 19
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RFP antibody is conjugated to Biotin
    Application
    • 27
    • 21
    • 15
    • 12
    • 10
    • 7
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))
    Cross-Reactivity (Details)
    Ads. to Hu, Ms, Rt Serum Proteins
    Purification
    Immunoaffinity Chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Immunogen
    Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma
    Isotype
    IgG
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  • Application Notes
    Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody can be used todetect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotinconjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP iswell suited to titrate RFP in solution. The detection antibody conjugated to biotin issubsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugatedpolyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cellexpression systems and can detect RFP containing inserts. Significant amplification ofsignal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to thefluorescence of RFP alone. For immunoblotting use either alkaline phosphatase orperoxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins onwestern blots. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry on Frozen Sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Comment

    Molar Ratio: 10-20 BAC molecules per IgG molecule

    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 0.1 mL of deionized water (or equivalent).
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA (IgG and Protease free)
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target
    RFP (Red Fluorescent Protein (RFP))
    Abstract
    RFP Products
    Background
    Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.Synonyms: DsRed Tag, Red fluorescent protein Tag
    Gene ID
    86600
    UniProt
    Q9U6Y8
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