BID antibody (BH3 Interacting Domain Death Agonist)

Details for Product anti-BID Antibody No. ABIN4284615
Antigen
  • FP497
  • 2700049M22Rik
  • AI875481
  • AU022477
  • BID
  • bid
  • si:ch211-238n5.6
  • fp497
  • xbid
  • DKFZp469E066
  • BH3 interacting domain death agonist
  • BH3 interacting domain death agonist S homeolog
  • BID
  • Bid
  • bida
  • bid.S
  • bid
Alternatives
anti-Human BID antibody for Immunohistochemistry (Paraffin-embedded Sections)
Reactivity
Dog (Canine), Gerbil, Human, Mouse (Murine), Rat (Rattus)
317
160
80
5
3
3
2
Host
Rabbit
310
76
21
6
Clonality
Polyclonal
Conjugate
This BID antibody is un-conjugated
26
21
13
8
7
7
6
6
6
6
6
6
3
3
3
3
3
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
328
166
152
75
47
41
29
18
15
11
10
9
3
2
1
1
1
1
1
Options
Immunogen Full-length recombinant mouse Bid protein was used as immunogen.
Specificity Full-length Bid is known to undergo cleavage/truncation (reviewed in Yin, 2006). Bid was initially found to be cleaved and activated by capspase-8 following death receptor activation. The term "Bid" was first used to described the caspase-8 cleaved/truncated C-terminal Bid. Bid can be also cleaved by other proteases such as Granzyme B, calpains and cathepsin. The C-terminal portion is considered to be the active Bid moiety. This active form can translocate from the cytosol to the mitochondria. Therefore, the appearance of Bid in the mitochondria is considered to be an indication of active Bid. However, it should also be noted that Bid has been shown to translocate to the mitochondria without cleavage in some model systems. The proteolytic cleavage of Bid usually occurs in the unstructured loop region between the alpha 2 and alpha 3 helices, which is between amino acids (aa) 41 and 79 of Bid. For example, the caspase-8/3 cleavage site is at 60 (human) and 59 (mouse). It should be noted that after cleavage, the smaller N-terminal portion of Bid is not necessarily separated from the larger C-terminal portion. The exact size of the C-terminal cleavage products depends on the Bid cleavage sites, generally 11-15 kDa C-truncated cleavage forms are generated. Shorter C-terminal Bid cleavage products may result from protease cleavage that involves both caspases and other proteases. Full length Bid is quite stable, but cleaved/truncated forms may have a short half-life (less than 1.5 h) and degrade rapidly. Therefore, a time course may be useful when detecting Bid cleavage fragments.
Purification Unpurified
Plasmids, Primers & others Plasmids, Primers & others BID products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name BID (BID Antibody Abstract)
Background Gene Symbol: BID
Gene ID 637
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
Application Notes Western Blot 1:1000-1:2000, Immunohistochemistry, Immunoprecipitation 1:50-1:200, Immunohistochemistry-Paraffin 1:1000-1:5000, Immunohistochemistry-Frozen 1:1000 - 1:5000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer Neat whole antisera
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-BH3 Interacting Domain Death Agonist (BID) antibody (ABIN4284615) Western Blot: BID Antibody [ABIN42846156] - Analysis of Bid in normal mouse tissues u...
Western Blotting (WB) image for anti-BH3 Interacting Domain Death Agonist (BID) antibody (ABIN4284615) Western Blot: BID Antibody [ABIN42846156] - Analysis of BID in NRK whole cell lysate ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BH3 Interacting Domain Death Agonist (BID) antibody (ABIN4284615) Immunohistochemistry-Paraffin: BID Antibody [ABIN42846156] - Formalin-fixed, paraffin...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BH3 Interacting Domain Death Agonist (BID) antibody (ABIN4284615) Immunohistochemistry-Paraffin: BID Antibody [ABIN42846156] - Two cores from a human o...
Product cited in: Liu, Yuan, Long, Luo, Bian, Liu, Gu, Zou, Song, Wang, Wang, Liu: "Beclin-1-mediated Autophagy Protects Against Cadmium-activated Apoptosis via the Fas/FasL Pathway in Primary Rat Proximal Tubular Cell Culture." in: Scientific reports, Vol. 7, Issue 1, pp. 977, 2017 (PubMed). (Sample species: Rat (Rattus)).

Wang, Parrish: "Loss of α(E)-catenin promotes Fas mediated apoptosis in tubular epithelial cells." in: Apoptosis : an international journal on programmed cell death, Vol. 20, Issue 7, pp. 921-9, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Tiao, Lin, Chen, Liou, Wang, Huang, Chou, Huang, Chuang: "Dexamethasone decreases cholestatic liver injury via inhibition of intrinsic pathway with simultaneous enhancement of mitochondrial biogenesis." in: Steroids, Vol. 76, Issue 7, pp. 660-6, 2011 (PubMed).

Krajewska, Rosenthal, Mikolajczyk, Stennicke, Wiesenthal, Mai, Naito, Salvesen, Reed, Fiskum, Krajewski: "Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation." in: Experimental neurology, Vol. 189, Issue 2, pp. 261-79, 2004 (PubMed). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

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