CD4 antibody (CD4 Molecule)

Details for Product anti-CD4 Antibody No. ABIN4292796
Antigen
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • carcinoembryonic antigen-related cell adhesion molecule 5
  • T-cell surface glycoprotein CD4
  • CD4 antigen
  • Cd4 molecule
  • CD4
  • LOC100125525
  • cd4
  • Cd4
Alternatives
anti-Human CD4 antibody for Flow Cytometry
Reactivity
Human
991
468
192
57
42
30
28
25
25
22
22
21
19
18
17
15
14
13
11
9
5
5
4
2
2
1
1
1
1
1
Host
Mouse
1075
386
216
8
2
2
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD4 antibody is un-conjugated
222
190
124
108
27
27
24
21
18
18
15
14
13
12
10
10
9
8
8
6
5
5
5
5
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Blocking Peptide (BP), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), In vitro Assay (in vitro), Neutralization (Neut)
1243
367
353
223
196
175
164
70
58
54
47
39
14
11
11
10
7
7
6
6
5
4
3
2
2
2
2
1
1
1
1
1
Options
Immunogen PHA-stimulated human PBMC.
Clone RPA-T4
Isotype IgG1 kappa
Purification Protein G purified
Plasmids, Primers & others Plasmids, Primers & others CD4 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background Gene Symbol: CD4
Gene ID 920
NCBI Accession NP_000607
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
Application Notes Flow Cytometry, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, In vitro assay, Blocking/Neutralizing 50-200 Molar excessRPA-T4 is capable of blocking HIV-1, gp120, and inhibits syncytium formation. The RPA-T4 clone reacts with CD4, a 59 kDa single-chain transmembrane glycoprotein [receptor for human HIV virus] present on T-helper/inducer cell populations. This antibody binds to the D1 domain of CDR1 and CDR3 epitopes. The CD4 antigen and reacts with approvimately 80 % of thymocytes and 45 % of peripheral blood lymphocytes. CD4 is also present in low density on peripheral blood monocytes. Clone RPA-T4 is widely published in literature, see Knapp W, Dorken B, Rieber E P, et all, ed. Also see Schlossman SF, Boumsell L, Gilks W, et al, ed.. Use in in vitro reported in multiple pieces scientific literature.

Not for use in: Immunohistochemistry-Paraffin
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer 0.2 mL PBS and 0.05 % BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 Molecule (CD4) antibody (ABIN4292796) Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Cell surface flow analysis of CD...
Flow Cytometry (FACS) image for anti-CD4 Molecule (CD4) antibody (ABIN4292796) Flow Cytometry: CD4 Antibody (RPA-T4) [NBP2-25199] - Analysis using Azide Free versio...
Product cited in: Hellwinkel, Redzic, Harland, Gunaydin, Anchordoquy, Graner: "Glioma-derived extracellular vesicles selectively suppress immune responses." in: Neuro-oncology, Vol. 18, Issue 4, pp. 497-506, 2016 (PubMed). Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Staal, Res, Weijer, Spits: "Development of retrovirally marked human T progenitor cells into mature thymocytes." in: International immunology, Vol. 7, Issue 8, pp. 1301-9, 1996 (PubMed).

Ruggiero, Martinez Cáceres, Voordouw, Noteboom, Graf, Kroczek, Spits: "CD40 expressed on thymic epithelial cells provides costimulation for proliferation but not for apoptosis of human thymocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 156, Issue 10, pp. 3737-46, 1996 (PubMed).

Res, Martínez-Cáceres, Cristina Jaleco, Staal, Noteboom, Weijer, Spits: "CD34+CD38dim cells in the human thymus can differentiate into T, natural killer, and dendritic cells but are distinct from pluripotent stem cells." in: Blood, Vol. 87, Issue 12, pp. 5196-206, 1996 (PubMed). (Sample species: Human). Further details: Flow Cytometry

Schols, De Clercq: "Human immunodeficiency virus type 1 gp120 induces anergy in human peripheral blood lymphocytes by inducing interleukin-10 production." in: Journal of virology, Vol. 70, Issue 8, pp. 4953-60, 1996 (PubMed). (Sample species: Human). Further details: In vitro Assay

Kaneko, Saito, Hashimoto, Yagita, Okumura, Azuma: "Preferential elimination of CD28+ T cells in systemic lupus erythematosus (SLE) and the relation with activation-induced apoptosis." in: Clinical and experimental immunology, Vol. 106, Issue 2, pp. 218-29, 1996 (PubMed).

Deng, Bell, Huie, Pinto, Hunt, Stinson, Sibley, Hall, Valantine: "Cardiac allograft vascular disease. Relationship to microvascular cell surface markers and inflammatory cell phenotypes on endomyocardial biopsy." in: Circulation, Vol. 91, Issue 6, pp. 1647-54, 1995 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Frozen Sections)

Aversa, Waugh, Hall: "A monoclonal antibody (A6) recognizing a unique epitope restricted to CD45RO and RB isoforms of the leukocyte common antigen family identifies functional T cell subsets." in: Cellular immunology, Vol. 158, Issue 2, pp. 314-28, 1994 (PubMed). (Sample species: Human). Further details: In vitro Assay

Bárcena, Muench, Galy, Cupp, Roncarolo, Phillips, Spits: "Phenotypic and functional analysis of T-cell precursors in the human fetal liver and thymus: CD7 expression in the early stages of T- and myeloid-cell development." in: Blood, Vol. 82, Issue 11, pp. 3401-14, 1994 (PubMed). (Sample species: Human). Further details: Flow Cytometry

Chabanne, Marchal, Kaplanski, Fournel, Magnol, Monier, Rigal: "Screening of 78 monoclonal antibodies directed against human leukocyte antigens for cross-reactivity with surface markers on canine lymphocytes." in: Tissue antigens, Vol. 43, Issue 3, pp. 202-5, 1994 (PubMed).

Galy, Verma, Bárcena, Spits: "Precursors of CD3+CD4+CD8+ cells in the human thymus are defined by expression of CD34. Delineation of early events in human thymic development." in: The Journal of experimental medicine, Vol. 178, Issue 2, pp. 391-401, 1993 (PubMed). (Sample species: Human). Further details: In vitro Assay,Flow Cytometry

Wilson, Crowley, Odgers, Shaw: "Immunofluorescent labeling using covalently linked anti-phycoerythrin antibodies and phycoerythrin polymers." in: Cytometry, Vol. 12, Issue 4, pp. 373-7, 1991 (PubMed).