CD74 antibody (HLA-DR-gamma)

Details for Product anti-CD74 Antibody No. ABIN4295634
Antigen
  • DHLAG
  • HLADG
  • II
  • Ia-GAMMA
  • CLIP
  • Ii
  • INVG34
  • Iclp-1
  • iclp1
  • wu:fb11f09
  • wu:fb12a01
  • wu:fb13d06
  • wu:fk94e07
  • CD74 molecule
  • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
  • CD74 molecule, major histocompatibility complex, class II invariant chain a
  • CD74
  • Cd74
  • cd74a
Reactivity
Baboon, Human, Mouse (Murine)
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103
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27
Host
Mouse
409
103
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD74 antibody is un-conjugated
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Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
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Options
Immunogen SU-DHL-4 lymphoma cells were used as the immunogen for the CD74 antibody
Clone LN-2
Isotype IgG1 kappa
Specificity It recognizes a protein of ~35 kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+). This antibody can be used for the identification of B cell lymphoma and leukemias. It also stains activated neoplastic cells in T cell lymphomas and Sternberg-reed cells.
No Cross-Reactivity Rat (Rattus)
Purification Protein G purified
Plasmids, Primers & others Plasmids, Primers & others CD74 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name CD74 (CD74 Antibody Abstract)
Background Gene Symbol: CD74
Molecular Weight Theoretical MW: 37 kDa
Gene ID 972
UniProt P04233
Pathways Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints
Application Notes Western Blot 0.5-1 μg/mL, Flow Cytometry 0.5-1 μg/million cells, ELISA 1-5 μg/mL for coating, Immunohistochemistry 0.5-1 μg/mL, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunoprecipitation 1-2 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 0.5-1 μg/mL, Immunohistochemistry-Frozen 0.5-1 μg/mLHuman Chromosome Location: 5q33.1 Use in Western Blot has been reported in literature. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.
Supplier Images
Image no. 1 for anti-HLA-DR-gamma (CD74) antibody (ABIN4295634) Immunohistochemistry-Frozen: CD74 Antibody (LN-2) [NBP2-29465] - Analysis using the A...
Product cited in: Zheng, Yang, Rong, Yuan, Ma, Wang, Shen, Cui: "CD74 and macrophage migration inhibitory factor as therapeutic targets in gastric cancer." in: World journal of gastroenterology : WJG, Vol. 18, Issue 18, pp. 2253-61, 2012 (PubMed).

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