Application Notes | Western Blot 1:100-1:500, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1 - 4 μg/mL, Immunohistochemistry-Paraffin 1:50-1:200For IHC-Paraffin HIER pH 6 retrieval is recommended. IF fixation permeabilization: PFA/Triton X-100. |
Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
Restrictions | For Research Use only |
Format | Liquid |
Buffer |
PBS ( pH 7.2) and 40 % Glycerol Buffer contains: 0.02 % Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Storage | 4 °C,-20 °C |
Storage Comment | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Supplier Images |
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Product cited in: |
De Marchi, Liu, Stingl, Timmermans, Smid, Look, Tjoa, Braakman, Opdam, Linn, Sweep, Span, Kliffen, Luider, Foekens, Martens, Umar: "4-protein signature predicting tamoxifen treatment outcome in recurrent breast cancer." in: Molecular oncology, Vol. 10, Issue 1, pp. 24-39, 2016 (PubMed).
Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human). Wei, Fattet, Tsai, Guo, Pai, Majeski, Chen, Sah, Taylor, Engler, Yang: "Matrix stiffness drives epithelial-mesenchymal transition and tumour metastasis through a TWIST1-G3BP2 mechanotransduction pathway." in: Nature cell biology, Vol. 17, Issue 5, pp. 678-88, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunohistochemistry |