Perforin 1 antibody (Perforin 1 (Pore Forming Protein)) (AA 402-534)

Details for Product anti-PRF1 Antibody No. ABIN4344818
Antigen
  • FLH2
  • HPLH2
  • P1
  • PFN1
  • PFP
  • PRF1
  • Pfn
  • Pfp
  • Prf-1
  • Cyta
  • RATCYTA
  • LOC443187
  • perforin
  • prf1
  • cytolysin
  • perforin-1
  • perforin-1-like
  • perforin 1
  • perforin 1 (pore forming protein)
  • perforin
  • perforin 1 L homeolog
  • PRF1
  • Prf1
  • LOC443187
  • prf1
  • prf1.L
Epitope
AA 402-534, AA 98-534
8
8
8
7
5
5
5
3
3
3
2
2
2
2
1
1
1
1
Reactivity
Mouse (Murine)
148
43
23
9
2
2
1
1
1
Host
Rat
98
76
9
Clonality (Clone)
Monoclonal ()
Conjugate
This Perforin 1 antibody is un-conjugated
19
16
9
4
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
99
66
41
40
37
19
18
17
16
13
8
4
2
2
2
1
1
1
1
1
1
Options
Immunogen Recombinant mouse perforin (aa 98-534).
Clone CB5-4
Isotype IgG2a
Specificity Recognizes mouse perforin (region aa 402-534).
Purification Protein G purified
Plasmids, Primers & others Plasmids, Primers & others Perforin 1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Perforin (PRF1 Antibody Abstract)
Background Gene Symbol: PRF1
Gene ID 5551
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Western Blot 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500This antbody is useful in Immunocytochemistry, Immunohistochemistry (frozen sections, paraffin sections), Immunoprecipitation and Western Blot.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 402-534), (AA 98-534) antibody (ABIN4344818) Flow Cytometry: Perforin Antibody (CB5.4) [NBP1-97512] - Analysis of 106 Jurkat cells...
Image no. 2 for anti-Perforin 1 (Pore Forming Protein) (PRF1) (AA 402-534), (AA 98-534) antibody (ABIN4344818) Western Blot: Perforin Antibody (CB5.4) [NBP1-97512] - Detection of perforin in the m...
Product cited in: Rah, Kwak, Chung, Kim: "ADP-ribose/TRPM2-mediated Ca2+ signaling is essential for cytolytic degranulation and antitumor activity of natural killer cells." in: Scientific reports, Vol. 5, pp. 9482, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Dalton, Howell, Pearson, Scott, Carding: "Fas-Fas ligand interactions are essential for the binding to and killing of activated macrophages by gamma delta T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 6, pp. 3660-7, 2004 (PubMed).

Crider-Pirkle, Billingsley, Faust, Hardy, Lee, Weitlauf: "Cubilin, a binding partner for galectin-3 in the murine utero-placental complex." in: The Journal of biological chemistry, Vol. 277, Issue 18, pp. 15904-12, 2002 (PubMed).

Müller, Tschopp: "Resistance of CTL to perforin-mediated lysis. Evidence for a lymphocyte membrane protein interacting with perforin." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 6, pp. 2470-8, 1994 (PubMed).

Dupuis, Schaerer, Krause, Tschopp: "The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes." in: The Journal of experimental medicine, Vol. 177, Issue 1, pp. 1-7, 1993 (PubMed).

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