TYRO3 antibody (TYRO3 Protein Tyrosine Kinase) (AA 100-250)

Details for Product anti-TYRO3 Antibody No. ABIN440474, Supplier: Log in to see
Antigen
  • TYRO3
  • brt
  • byk
  • dtk
  • rse
  • sky
  • tif
  • Xksy
  • MGC145734
  • DKFZp469I0432
  • LOC100231598
  • BYK
  • Dtk
  • RSE
  • Sky
  • Tif
  • Brt
  • REK
  • ksy
  • xksy
  • AI323366
  • Etk-2
  • Rse
  • TYRO3 protein tyrosine kinase
  • TYRO3 protein tyrosine kinase L homeolog
  • TYRO3 protein tyrosine kinase 3
  • TYRO3
  • tyro3
  • Tyro3
  • tyro3.L
Alternatives
anti-Human TYRO3 antibody for ELISA
Epitope
AA 100-250, Internal Region
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Reactivity
Human, Mouse (Murine)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This TYRO3 antibody is un-conjugated
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Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen Synthetic peptide made to an internal portion of the human TYRO3 protein (within residues 100-250). [Swiss-Prot# Q06418]
Purification Immunogen affinity purified
Plasmids, Primers & others Plasmids, Primers & others TYRO3 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Dtk/TYRO3 (TYRO3 Antibody Abstract)
Background Gene Symbol: TYRO3
Molecular Weight Theoretical MW: 96 kDa
Gene ID 7301
UniProt Q06418
Pathways RTK Signaling
Application Notes Western Blot 2 μg/mL, Flow Cytometry, Immunocytochemistry/Immunofluorescence 1:200This TYRO3 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~96 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in FLOW cytometry reported in scientific literature ( PMID 27034374).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for TYRO3 Antibody Procedure Guide for NBP1-28635 - Tyro3 AntibodyProcedure Guide for NBP1-28635- Tyro3 AntibodyWestern Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer ofproteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locationsand locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Dilute the rabbit anti-Tyro3 primary antibody in blocking buffer and incubate 1 hour at roomtemperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated asrequired to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, providedit does not interfere with antibody-antigen binding.(c) 2009 Novus Biologicals - Tyro3 Antibody - Page 1
Restrictions For Research Use only
Format Liquid
Concentration 1.19 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-TYRO3 Protein Tyrosine Kinase (TYRO3) (AA 100-250), (Internal Region) antibody (ABIN440474) anti-TYRO3 Protein Tyrosine Kinase (TYRO3) (AA 100-250), (Internal Region) antibody
Immunofluorescence (IF) image for anti-TYRO3 Protein Tyrosine Kinase (TYRO3) (AA 100-250), (Internal Region) antibody (ABIN440474) Immunocytochemistry/Immunofluorescence: Dtk/TYRO3 Antibody [NBP1-28635] - Staining of...
Flow Cytometry (FACS) image for anti-TYRO3 Protein Tyrosine Kinase (TYRO3) (AA 100-250), (Internal Region) antibody (ABIN440474) Flow Cytometry: Dtk/TYRO3 Antibody - An intracellular stain was performed on HeLa ce...
Product cited in: Chan, Carrera Silva, De Kouchkovsky, Joannas, Hao, Hu, Huntsman, Eng, Licona-Limón, Weinstein, Herbert, Craft, Flavell, Repetto, Correale, Burchard, Torgerson, Ghosh, Rothlin: "The TAM family receptor tyrosine kinase TYRO3 is a negative regulator of type 2 immunity." in: Science (New York, N.Y.), Vol. 352, Issue 6281, pp. 99-103, 2016 (PubMed). Further details: Flow Cytometry

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