HNF1 Homeobox A (HNF1A) (Center) antibody

Details for Product No. ABIN443456
Target
Binding Specificity
Center
15
12
8
4
4
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse, Rat
74
51
35
8
7
7
6
5
3
2
2
2
1
1
1
Host
Rabbit
66
8
1
Clonality
Polyclonal
Conjugate
Un-conjugated
5
4
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
45
20
13
11
11
5
3
3
2
2
2
2
1
1
1
Options
Immunogen Recombinant protein encompassing a sequence within the center region of human HNF1 alpha. The exact sequence is proprietary.
Isotype IgG
Specificity Human HNF-1 alpha protein.
Characteristics Sample: HeLa cells were fixed in 4 % paraformaldehyde at RT for 15 min. Green: HNF1 alpha protein stained by HNF1 alpha antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (diluted at 1:1000. Blue: Hoechst 33342 staining.
Purification Immunogen affinity purified
Target
Alternative Name HNF1 (HNF1A Antibody Abstract)
Background Gene Symbol: HNF1A
Molecular Weight Theoretical MW: 67 kDa
Gene ID 6927
UniProt P20823
Pathways Hormone Transport, Carbohydrate Homeostasis
Application Notes Western Blot 1:1000-1:10000, Immunohistochemistry 1:100-1:1000, Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunoprecipitation 1:100-1:500, Immunohistochemistry-Paraffin 1:100-1:1000The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS ( pH 7.0) and 20 % Glycerol
Buffer contains: 0.01 % Thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Images
Image no. 1 for anti-HNF1 Homeobox A (HNF1A) (Center) antibody (ABIN443456) Western Blot: HNF1 Antibody - Various whole cell extracts (30 ug) were separated by ...
Product cited in: Bisceglia, Battistelli, Noce, Montaldo, Zammataro, Strippoli, Tripodi, Amicone, Marchetti: "TGFβ Impairs HNF1α Functional Activity in Epithelial-to-Mesenchymal Transition Interfering With the Recruitment of CBP/p300 Acetyltransferases." in: Frontiers in pharmacology, Vol. 10, pp. 942, 2019 (PubMed). Method employed by authors: Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC), Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)

Yasui, Miyoshi, Tababe, Ishigami, Fukutomi, Imai, Isemura: "Effects of oolong tea on gene expression of gluconeogenic enzymes in the mouse liver and in rat hepatoma H4IIE cells." in: Journal of medicinal food, Vol. 14, Issue 9, pp. 930-8, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting

Yasui, Tanabe, Miyoshi, Suzuki, Goto, Taguchi, Ishigami, Paeng, Fukutomi, Imai, Isemura: "Effects of (-)-epigallocatechin-3-O-gallate on expression of gluconeogenesis-related genes in the mouse duodenum." in: Biomedical research (Tokyo, Japan), Vol. 32, Issue 5, pp. 313-20, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting