His Tag antibody

Details for Product No. ABIN449792, Supplier: Log in to see
Antigen
Reactivity
Tag
625
28
15
3
3
Host
Goat
415
187
26
23
8
8
3
2
Clonality
Polyclonal
Conjugate
Un-conjugated
58
46
36
17
14
13
10
10
9
9
9
9
8
8
8
7
7
7
5
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
564
402
163
107
79
78
74
26
13
11
9
7
6
6
6
5
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Keyhole limpet haemocyanin (KLH) conjugated H-H-H-H-H-H
Isotype IgG
Specificity This antibody recognizes the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. This antibody may not detect all N-terminal (NT) histidine tagged proteins.
Purification Immunogen affinity purified
Alternative Name 6xHistidine Epitope Tag (HHHHHH)
Target Type Tag
Research Area Tags/Labels
Application Notes Western Blot 1:1000-1:10000, ELISA 1:1000 - 1:30000, Immunocytochemistry/Immunofluorescence 1:100-1:400, Immunoprecipitation 1 - 4 μg/mg lysate
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-His Tag antibody (ABIN449792) Western Blot: 6-Histidine Epitope Tag Antibody [NBP1-25939] - 200, 100, or 50 ng of E...
Product cited in: Cabrita, Jäggi, Widjaja, Christofori: "A functional interaction between sprouty proteins and caveolin-1." in: The Journal of biological chemistry, Vol. 281, Issue 39, pp. 29201-12, 2006 (PubMed).

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