CAMK2A antibody (Calcium/calmodulin-Dependent Protein Kinase II alpha) (pThr286)

Details for Product anti-CAMK2A Antibody No. ABIN451525
Antigen
Epitope
pThr286, pThr287
24
22
12
12
12
12
10
8
7
5
5
3
3
3
2
2
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
148
97
94
5
4
3
2
1
1
1
1
1
1
1
Host
Mouse
147
34
7
Clonality (Clone)
Monoclonal ()
Conjugate
This CAMK2A antibody is un-conjugated
11
8
8
6
5
5
5
5
5
3
3
3
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
150
71
41
35
25
21
12
11
8
5
5
5
2
1
Options
Immunogen Synthetic peptide and it detects phosphorylated CaMKII from rat tissues.
Clone 22B1
Isotype IgG1
Specificity This antibody is specific for a and B subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in B).
Purification Protein G purified
Antigen
Alternative Name CAMK2A (CAMK2A Antibody Abstract)
Background Gene Symbol: CAMK2A
Gene ID 815
UniProt P11275
Pathways WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction
Application Notes Western Blot 1 μg/mL, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:5001 μg/mL was sufficient for detection of 0.2 μg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS ( pH 7.4) and 50 % Glycerol
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Image no. 1 for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr286), (pThr287) antibody (ABIN451525) Western Blot: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [ABIN4515259] - ...
Image no. 2 for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr286), (pThr287) antibody (ABIN451525) Immunohistochemistry-Paraffin: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1)...
Image no. 3 for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr286), (pThr287) antibody (ABIN451525) Immunohistochemistry: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1) [ABIN451...
Image no. 4 for anti-Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A) (pThr286), (pThr287) antibody (ABIN451525) Immunohistochemistry-Paraffin: CaMKII alpha/beta [p Thr286, p Thr287] Antibody (22B1)...
Product cited in: Cai, Kasikara, Doran, Ramakrishnan, Birge, Tabas: "MerTK signaling in macrophages promotes the synthesis of inflammation resolution mediators by suppressing CaMKII activity." in: Science signaling, Vol. 11, Issue 549, 2018 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Ersoy, Maner-Smith, Li, Alpertunga, Cohen: "Thioesterase-mediated control of cellular calcium homeostasis enables hepatic ER stress." in: The Journal of clinical investigation, Vol. 128, Issue 1, pp. 141-156, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Conlon, Thomas, Fedotova, Hernandez-Ono, Di Paolo, Chan, Ruggles, Gibeley, Liu, Ginsberg: "Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis." in: The Journal of clinical investigation, Vol. 126, Issue 10, pp. 3852-3867, 2016 (PubMed).

Jiang, Xu, Song, Li, Mao, Zhao, He, Yang, Dai: "Calmodulin-dependent protein kinase II/cAMP response element-binding protein/Wnt/β-catenin signaling cascade regulates angiotensin II-induced podocyte injury and albuminuria." in: The Journal of biological chemistry, Vol. 288, Issue 32, pp. 23368-79, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting